BD Horizon™ Fixable Viability Stain 700 564997

BD Horizon™ Fixable Viability Stain 700 564997

BD Horizon™ Fixable Viability Stain 700 (FVS700) is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. This dye reacts with and covalently binds to cell-surface and intracellular amines. Permeable plasma cell membranes, such as those present in necrotic cells, allow for the intracellular diffusion and covalent binding of the dye to the higher levels of total cellular amines when compared to living cells with impermeable plasma membranes.  Therefore, necrotic cells present in a typical in vitro assay label with higher levels of dye increasing their FVS700 fluorescence intensities 10-20 fold over that of viable cells. The FVS700-labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.

BD Horizon™ Fixable Viability Stain 700 is excited by the Red laser (with an excitation maximum of 657 nm), and has a fluorescence emission maximum of 700 nm.

Flow cytometric analysis of human Jurkat cells stained with BD Horizon™ Fixable Viability Stain 700. Cells from the human Jurkat (Acute T cell leukemia, ATCC TIB-152) cell line were treated with 0.025% DMSO (Left Panel) or 5 μM camptothecin (Right Panel) for 16 hours and then stained with BD Horizon™ Fixable Viability Stain 700 (Cat. No. 564997) in serum-free buffer. The cells were then either left unfixed (solid line histograms) or fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized in BD Phosflow™ Perm/Wash Buffer I (Cat. No. 557885, dashed line histograms). Histograms were derived from gated events with the forward and side light-scattering characteristics of Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometry System. Please note that FVS700 is also compatible with BD Phosflow™ Perm Buffer III (Cat. No.558050) or BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). Fixable Viability Stain 700 has been tested on mouse (data not shown).