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PRO-284

人血管抑素纖維蛋白溶酶原（rHuAng K1-3）

20μg/100μg/1mg

Prospec

Background:
	Angiostatin was observed initially as an angiogenesis inhibitor, specifically inhibiting endothelial cell proliferation Recombinant angiostatin with one less kringle domain （kringle 1-3） acts as an effective inhibitor of angiogenesis induced by angiogenin , FGF basic, and VEGF
	Description :
	Recombinant Human Angiostatin produced in Pichia Pastoris is a single, non-glycosylated polypeptide chain having a molecular mass of 29711 Dalton. rHuAng K1-3 is a proteolytic fragment of plasminogen containing the first three kringle structures. A specific inhibitor of endothelial cell growth and angiogenesis. More active relative to kringles 1-4. rHuAng K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. Human Angiostatin is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered liquid formulation.
	Formulation:
	1mg/ml containing 0.5X PBS.
	Stability:
	Recombinant Human Angiostatin K1-3 should be stored at 4⁰c. For long term storage it is recommended to add a carrier protein （ % HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	Inhibitory activity （ED50=70 nm） relative to Kringles 1-4 （ED50=135 nm）. Kringles 1-3 acts as inhibitor of endothelial cell growth and angiogenesis.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of Recombinant Angiostatin.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.08 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Angiostatin as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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