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CYT-333

重組人骨形態發生蛋白7（rHuBMP-7）

2μg/10μg/1mg

Prospec

	Background :
	BMP-7 （osteogenic protein 1） belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta （TGF-beta） superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.
	Description :
	Recombinant Human BMP-7 produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 139 amino acids and having a molecular mass of 15679.97 Dalton. BMP-7 is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	BMP-7 was lyophilized from a concentrated （1mg/ml） sterile solution containing 10mM sodium citrate pH=3.5.
	Solubility:
	It is recommended to reconstitute the lyophilized rHuBMP-7 in 40mM acetic acid not less than 100µg/ml .
	Stability:
	Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BMP-7 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Thr-Gly-Ser-Lys.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Applications:
	1.Molecular standard （Western, ELISA） in studying secreted BMP-7 2.Preparing antibodies for BMP-7 monomer. 3.Molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of Bone Morphogenetic protein-7 .
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm 2. Analysis by RP-HPLC, using a standard solution of BMP-7 as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.