聯系電話
上海起發實驗試劑有限公司
中級會員·16年
- 聯系人：
- 楊經理
- 電話：
- 021-50724187 021-50724961
- 手機：
- 15921799099
- 傳真：
- 021-50724961
- 地址：
- 上海浦東川沙鎮川沙路6619號上海起發實驗試劑有限公司
- 個性化：
- www.qfbio.com
- 網址：
- www.qfbio.com
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Gene Bridges - 重組公司
使用Red / ET重組工程允許在任何選定位置克隆，亞克隆和修飾DNA。它允許設計任何大小的DNA分子，包括非常大的DNA分子，如BAC或大腸桿菌染色體。
紅/ ET重組增加了CRISPR / Cas9的功效以擴展基因組研究。作為歐盟資助項目AgedBrainSYSBIO的一部分，我們制定了一項新的人類化大型基因組區域戰略。通過將CRISPR / Cas9與Red / ET Recombineering組合，我們成功地將 33kb人片段敲入小鼠ApoE基因座。
4-Way Recombineering（4WR）提供了一種新的快速簡化的方法，可在單個體內重組步驟中從多4個DNA片段（例如PCR產物）組裝質粒。4WR是Gene Bridges廣受歡迎的直接克隆 - 大腸桿菌菌株GB05-dir（目錄號K008）的新應用。
Gene Bridges擁有Red / ET Recombination的專有權，并被允許頒發技術許可并提供重組工程服務。沒有其他學術或商業組織有權提供重組工程服務。
genebridges2019年價格表
| 貨號 | 品名 | 規格 | 價格 |
| K001 | * Quick & Easy BAC Modification Kit | Kit | 13900 |
| K002 | * Counter Selection BAC Modification Kit | Kit | 16400 |
| K003 | * BAC Subcloning Kit | Kit | 13900 |
| K004 | * Quick & Easy Conditional Knock Out Kit - FRT | Kit | 13900 |
| K005 | * Quick & Easy Conditional Knock Out Kit - loxP | Kit | 13900 |
| K006 | *Quick & Easy E.Coli Gene Deletion Kit | Kit | 13900 |
| K008 | *Direct-cloning-proficient E.coli strain GB05-dir | Kit | 12400 |
| K009 | *Recombineering-proficient E.coli strain GB08-red | Kit | 12400 |
| A001 | Pro- and Eukaryotic Neomycin Selection Cassette (PGK-gb2-neo) | 50ng/ | 5980 |
| A002 | FRT flanked, Pro and Euk. Neomycin Select. Cassette (FRT-PGK-gb2-neo-FRT) | 50ng/ | 5980 |
| A003 | loxP flanked, Pro and Euk. Neomycin Select. Cassette (loxP-PGK-gb2-neo-loxP) | 50ng/ | 5980 |
| A004 | FRT flanked, Pro and Euk. Neomycin Select. Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) | 50ng/ | 5980 |
| A005 | FRT flanked, Pro and Euk. Neomycin Select. Cassette plus loxP site 2nd version (loxP-FRT-PGK-gb2-neo-FRT) | 50ng/ | 5980 |
| A006 | FRT flanked Chloramphenicol Select. Cassette (FRT-cm-FRT) | 50ng/ | 5980 |
| A007 | loxP flanked Chloramphenicol Select. Cassette (loxP-cm-loxP) | 50ng/ | 5980 |
| A008 | FRT flanked Ampicillin Select. Cassette (FRT-amp-FRT) | 50ng/ | 5980 |
| A009 | loxP flanked Ampicillin Select. Cassette (loxP-amp-loxP) | 50ng/ | 5980 |
| A010 | FRT flanked, Pro and Euk. Hygromycin Select. Cassette (FRT-PGK-gb2-hygro-FRT) | 50ng/ | 5980 |
| A011 | loxP flanked, Pro and Euk. Hygromycin Select. Cassette (loxP-PGK-gb2-hygro-loxP) | 50ng/ | 5980 |
| A012 | iCre-FRT-neo-FRT (codon improved Cre(iCre) with attached FRT flanked, Pro- and Eukaryotic Neomycin selection cassette | 50ng/ | 6500 |
| A013 | iCreERT2-FRT-neo-FRT (ligand-inducible iCre with attached FRT flanked, Pro- and Eukaryotic Neomycin Selection cassette) | 50ng/ | 6500 |
| A104 | Enhanced Flp Expression Plasmid:707-FLPe; tet | 0.2ug/ | 5980 |
| A105 | Enhanced Flp Expression Plasmid:708-FLPe;cm | 0.2ug/ | 5980 |
| A106 | Enhanced Flp Expression Plasmid:709-FLPe;amp | 0.2ug/ | 5980 |
| A107 | Enhanced Flp Expression Plasmid:710-FLPe;km | 0.2ug/ | 5980 |
| A112 | Cre Expression Plasmid:705-Cre;cm | 0.2ug/ | 5980 |
| A113 | Cre Expression Plasmid:706-Cre;tet | 0.2ug/ | 5980 |
| A114 | Cre Expression Plasmid:704-Cre;amp | 0.2ug/ | 5980 |
| A201 | Enhanced Eukaryotic FLP Expression Plasmid: pCAGGS-FLPe (Academia) | 0.2ug/ | 7500 |
| A203 | Eukaryotic FLPo Recombinase Expression Plasmid: pCAGGS-FLPo (Academia)* | 0.2ug/ | 7500 |
| A204 | Eukaryotic Cre Recombinase Expression Plasmid: pCAGGS-Cre (Academia)* | 0.2ug/ | 7500 |
| A205 | Eukaryotic Der Recombinase Expression Plasmid: CAGGS-Dre(Academia)* | 0.2ug/ | 7500 |
| A301 | Arabinose-inducible Prokaryotic Cre Recombinase Expression Plasmid: pSC101-BAD-Cre | 0.2ug/ | 5980 |
| A302 | Arabinose-inducible Prokaryotic Dre Recombinase Expression Plasmid: pSC101-BAD-Dre* | 0.2ug/ | 5980 |
| A303 | Arabinose-inducible Prokaryotic FLPe Recombinase Expression Plasmid: pSC101-BAD-FLPe (tet) | 0.2ug/ | 5980 |
