聯系電話
上海起發實驗試劑有限公司
中級會員·16年
- 聯系人：
- 楊經理
- 電話：
- 021-50724187 021-50724961
- 手機：
- 15921799099
- 傳真：
- 021-50724961
- 地址：
- 上海浦東川沙鎮川沙路6619號上海起發實驗試劑有限公司
- 個性化：
- www.qfbio.com
- 網址：
- www.qfbio.com
掃一掃訪問手機商鋪
 提 供 商 |
上海起發實驗試劑有限公司 |  資料大小 |
193.8KB |
|---|---|---|---|
 資料類型 |
PDF 文件 |  資料圖片 |
|
 下載次數 |
282次 |  瀏覽次數 |
2536次 |
| 型號 | 載體名稱 | 出品公司 | 載體用途 | 價格 |
| VPP0042 | pCI-neo | Promega | 哺乳動物表達載體 | 800 |
產品參數:
Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：CMV
Cloning Method：Restriction Enzyme/MCS
Constitutive or Inducible System：Constitutive
載體抗性:
氨芐青霉素（Ampicillin）
載體描述:
The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus （CMV） immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. This vector also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. （1）Strong, Constitutive Expression: The human cytomegalovirus （CMV） immediate-early enhancer/promoter region produces strong, constitutive expression. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. The vector is maintained as an episome in cells expressing the SV40 large T antigen, leading to even higher levels of expression. （2）Transient or Stable Expression: The neomycin phosphotransferase gene allows selection of stable transfected cells. （3）Increased Steady-State mRNA Levels: The late SV40 polyadenylation signal increases the steady-state level of RNA approximay fivefold more than the early SV40 polyadenylation signal. （4）Convenient: Multiple cloning sites exist for easy insertion of cDNA. （5）Versatile: Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication.
質粒圖譜:
上海起福生物科技有限公司
：楊建輝
：
辦公：
*8006
:
:http://www.qfbio.com/
地址：上海市浦東新區繡川路561號1102室
