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【文獻標題】Course-, dose-, and stage-dependent toxic effects of prenatal dexamethasone exposure on fetal articular cartilage development

【作者】Ze Chen，Zhe Zhao，Yunzepeng Li，et.al

【作者單位】武漢大學(xué)基礎(chǔ)醫(yī)學(xué)院（Basic Medical School of Wuhan University）

【文獻中引用產(chǎn)品】

試劑-藏紅O

【關(guān)鍵詞】Prenatal exposure，Dexamethasone，Developmental toxicity，Articular cartilage development，Transforming growth factor beta

【DOI】doi.org/10.1016/j.toxlet.2018.01.008

【影響因子(IF)】5.70

【出版期刊】《Toxicology Letters》

【產(chǎn)品原文引用】

Dexamethasone (CAS. NO. 50-02-2, 1 ml: 5 mg, AR) was obtained from Kingyork Group Co., Ltd. (Tianjin, China). afranin-O (CAS. NO.477-73-6) was obtained from Hengyuan Biotech Co., Ltd. (Shanghai,China). Monoclonal mouse collagen type II alpha 1 (Col2a1) antibody (sc-52658, 100 μg/ml) was purchased from Santa Cruz Biotechnology,Inc. (Santa Cruz, CA, USA). Trizol reagent kits were obtained from Omega Bio-Tek (Doraville, USA). The SYBR Green dye was purchased from ABI (Foster City, CA, USA). Reverse transcription and real-time quantitative polymerase chain reaction (RT-qPCR) kits were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). The oligonucleotide primers for mouse RT-qPCR genes (PAGE purification) were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). Other chemicals and agents were of analytical grade.

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