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excellness代理,PrimeCoat彈性基底培養皿板

PrimeCoat series
 

The ExCellness PrimeCoat series is designed specifically to provide a biomimetic cell culture environment that improves cell characteristics and phenotype in laboratory applications.

Key features

• PrimeCoat elastic substrates are easy to use for cell culture and subsequent analysis.
• PrimeCoat elastic substrates are available with 6 degrees of softness within the elasticity range of body tissues: 2, 5, 10, 15, 30 and 100 kPa.
• PrimeCoat elastic substrates are available in 4 standard formats: 100 mm diameter dishes, 40 mm diameter dishes, 24-well plates and 20x20 mm cover slips.
• PrimeCoat elastic substrates require to be coated by the end user to promote cell adhesion.
• PrimeCoat elastic substrates are transparent. Cells can be visualized with standard transmission light microscopes (e.g., Phase contrast, DIC).
• PrimeCoat elastic substrates are compatible with most standard molecular or cellular techniques: immunofluorescence, immunohistochemistry, protein analysis (e.g., Western blotting, and RNA/DNA extraction).

Biomimetic cell culture substrates at reach

PrimeCoat series combines simplicity and accessibility:

• simplicity of cell handling
• straightforward compatibility with cell analysis tools
• accessible prices

ExCellness in selected peer reviewed publications

In the following peer-reviewed publications, ExCellness biomimetic cell culture devices have been used or cited:

• A Glentis et al. Cancer-associated fibroblasts induce metalloprotease-independent cancer cell invasion of the basement membrane. Nat Commun. 2017 Oct 13;8(1):924.
• N Vedrenne et al. Isolation of astrocytes displaying myofibroblast properties and present in multiple sclerosis lesions. Neurochem. Res. 2017 Apr 22. 
• X L Chen et al. MicroRNA-21 preserves the fibrotic mechanical memory of mesenchymal stem cells. Nature Materials 16, 379–389 (2017).
• MR Zeglinski et al. Chronic expression of Ski induces apoptosis and represses autophagy in cardiac myofibroblasts. Biochim.  Biophys. Acta. 2016 Jun;1863(6 Pt A):1261-8.
• H Chen et al. Mechanosensing by the α6-integrin confers an invasive fibroblast phenotype and mediates lung fibrosis. Nat. Commun. 2016 Aug 18;7: 12564.
• NP Talele et al. Expression of α-Smooth Muscle Actin Determines the Fate of Mesenchymal Stromal Cells. Stem Cell Reports. 2015 Jun 9;4(6) : 1016-30.

• V Sarrazy et al. Integrins avb5 and avb3 promote latent TGF-beta 1 activation by human cardiac fibroblast contraction. Cardiovasc Res (2014) 102 (3)
• VF Achterberg et al. The nano-scale mechanical properties of the extracellular matrix regulate dermal fibroblast function.J Invest Dermatol. 2014 Jul;134(7):1862-72.
• JA Cadby et al. Differences between the Cell Populations from the Peritenon and the Tendon Core with Regard to Their Potential Implication in Tendon Repair. 2014. PLoS ONE 9(3): e92474.
• T. Grand et al. Aggravation of Cardiac Myofibroblast Arrhythmogeneicity by Mechanical Stress. Cardiovasc Res. 2014 Dec 1;104(3):489-500.
• JA Cadby. Can we improve tendon healing in the horse? A multi-angle study of a multi-facet problem. ISBN: 978-90-5335-715-6.
• A Vashist et al. Recent advances in hydrogel based drug delivery systems for the human body. J. Mater. Chem. B, 2014,2, 147-166.
• EP van der Veer et al. The RNA-Binding Protein Quaking is a Critical Regulator of Vascular Smooth Muscle Cell Phenotype. Circ Res. 2013 Oct 12;113(9):1065-75.
• A De Boeck et al. Differential secretome analysis of cancer-associated fibrobroblasts and bone marrow-derived precursors toidentify microenvironmental regulators of colon cancerprogression. Proteomics 2013,13,379-388.
• C Godbout et al. The Mechanical Environment Modulates Intracellular Calcium Oscillation Activities of Myofibroblasts.PLoS One. 2013; 8(5): e64560.
• S Constant et al. Colon Cancer: Current Treatments and Preclinical Models for the Discovery and Development of New Therapies. Drug discovery; Editor Hany A. El-Shemy. ISBN 978-953-51-0906-8.
• JL Balestrini et al. The mechanical memory of lung myofibroblasts. Integr. Biol., 2012,4, 410-421.
• Stem Cells and Cancer Stem Cells, Volume 8. Therapeutic Applications in Disease and Injury. Editors M.A. Hayat. ISBN 978-94-007-4797-5.

• A Skardal et al. Bioprinted amniotic fluid-derived stem cells accelerate healing of large skin wounds. Stem Cells Transl Med. 2012 Nov;1(11):792-802. doi: 10.5966/sctm.2012-0088. Epub 2012 Oct 29.
• A Skardal et al. Substrate elasticity controls cell proliferation, surface marker expression and motile phenotype in amniotic fluid-derived stem cells. J Mech Behav Biomed Mater. 2013 January; 17: 307-316.
• BJ Crider et al. Myocardin-Related Transcription Factors A and B Are Key Regulators of TGF-b1-Induced Fibroblast to Myofibroblast Differentiation. Journal of Investigative Dermatology (2011) 131, 2378-2385.
• L Follonier Casla et al. A new lock-step mechanism of matrix remodelling based on subcellular contractile events. Journal of Cell Science 123, 1751-1760.

訂貨信息：

Dishes
Product Code     Description     
01. 100.002.00     2 kPa culture surface 100 mm ø     
01. 100.005.00     5 kPa culture surface 100 mm ø     
01. 100.010.00     10 kPa culture surface 100 mm ø     
01. 100.015.00     15 kPa culture surface 100 mm ø     
01. 100.030.00     30 kPa culture surface 100 mm ø     
01. 100.100.00     100 kPa culture surface 100 mm ø     

Product Code     Description     
01. 035.002.00     2 kPa culture surface 35 mm ø     
01. 035.005.00     5 kPa culture surface 35 mm ø     
01. 035.010.00     10 kPa culture surface 35 mm ø     
01. 035.015.00     15 kPa culture surface 35 mm ø     
01. 035.030.00     30 kPa culture surface 35 mm ø     
01. 035.100.00            100 kPa culture surface 35 mm ø 
 
Multi-well plates 

Product Code     Description     
04.024.002.00     2 kPa culture surface 24-well plate     
04.024.005.00     5 kPa culture surface 24-well plate     
04.024.010.00     10 kPa culture surface 24-well plate     
04.024.015.00     15 kPa culture surface 24-well plate     
04.024.030.00     30 kPa culture surface 24-well plate     
04.024.100.00     100 kPa culture surface 24-well plate 

    
Cover slips

Product Code     Description 

03.020.002.00     2 kPa culture surface 20x20 mm c.slip 
03.020.005.00     5 kPa culture surface 20x20 mm c.slip 
03.020.010.00     10 kPa culture surface 20x20 mm c.slip 
03.020.015.00     15 kPa culture surface 20x20 mm c.slip 
03.020.030.00     30 kPa culture surface 20x20 mm c.slip