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The SuperSense™ Methylated DNA Quantification Kit is a complete set of essential components which enables the experimenter to fluorometrically measure levels of methylated DNA using a small amount of DNA samples. The SuperSense™ Methylated DNA Quantification Kit is suitable for detecting global DNA methylation status using genomic DNA isolated from any species such as mammal, plant, fungus, bacteria and virus in a variety of forms including cultured cells, fresh and frozen tissues, paraffin-embedded tissues, plasma/serum samples, and body fluid samples, etc.

WHY CHOOSE THE SUPERSENSE™ METHYLATED DNA QUANTIFICATION KIT?

• Fluorescence-based assay with easy-to-follow steps for convenience and speed. The whole procedure can be finished within 2 hours and 30 minutes.
• High sensitivity, of which detection limit can be as low as 50 pg of methylated DNA with the input DNA being as low as 2 ng.
• Universal positive control, which is suitable for quantifying methylated DNA from any species.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.

The SuperSense™ Methylated DNA Quantification Kit contains all reagents required for fluorescent quantification of global DNA methylation. In this assay, DNA is immobilized to the strip well specifically coated with a DNA affinity substance. The methylated DNA can be recognized with anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to fluorescence intensity, can then be fluorometrically quantified.

 

SCHEMATIC PROCEDURE:
	Quantification of methylated DNA using the fluorometric SuperSense Methylated DNA Quantification Kit.

SA1 （10X Wash Buffer）
SA2 （DNA Binding Solution）
SA3 （Positive Control, 100 µg/ml）**
SA4 （Block Solution）
SA5 （Capture Antibody, 1000 µg/ml）*
SA6 （Labeling Solution）*
SA7 （Fluoro Developer）*
SA8 （Fluoro Enhancer）*
SA9 （Fluoro Dilution）
8-Well Assay Strips （with Frame）
User Guide

* Spin the solution down to the bottom before use.
** This control is synthesized polynucleotide methylated at every 5-cytosine.

Wong, CP. et. al. （September 2011）. Induction of regulatory T cells by green tea polyphenol EGCG. Immunol Lett. 139（1-2）:7-13.PubMed Abstract

Geyer, K. et. al. （August 2011）. Cytosine methylation regulates oviposition in the pathogenic blood fluke Schistosoma mansoni. Nat Commun. 2:424. PubMed Abstract

Cho, YH. et. al. （July 2011）. Identification of transcriptional regulatory elements required for the Mup2 expression in circadian clock mutant mice. Biochem Biophys Res Commun. 410（4）:834-40. PubMed Abstract

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by ing info@epigentek.com along with your contact information and institution name.