invitrogen Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation
貨號：貨號11456D  包裝0.4 ml  市場價格：1204元
貨號：貨號11452D  包裝2 ml  市場價格：6130
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Description
Dynabeads® Mouse T-Activator CD3/CD28 is for the activation and expansion of mouse T cells for research purposes.
With Dynabeads® Mouse T-Activator CD3/CD28 you get:
1）Activation of T cells without the need of feeder cells
2）Activated cells that retain in vivo-like function
3）Maximal reproducibility without contamination by soluble antibodies or mitogens
4）From 100- to 1000-fold T cell expansion
How Dynabeads® Mouse T-Activator CD3/CD28 works:
Dynabeads® Mouse T-Activator CD3/CD28 offers a simple method for activation and expansion of T cells that does not require feeder cells （antigen-presenting cells） or antigen. The 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T-cell activation and expansion. Expansion of the T-cell population can be stimulated using recombinant IL-2, and View Moreafter activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet.
For expansion of antigen specific T cells from T cell clones or T cell lines, we recommend using Dynabeads® Mouse T-Activator CD3/CD28/CD137.
Starting samples
Start with single cell suspensions from spleen or lymph nodes, mononuclear cells （MNCs）, whole blood, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells. For an overview over the different Dynabeads® available for cell isolation, look here.

Downstream applications
The activated T cells can be analyzed shortly after activation （for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.）. T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.
For research use only. Not for use in diagnostic procedures.
Contents and Storage
Dynabeads® Mouse T-Activator CD3/CD28 are supplied as a suspension containing 4 × 107 Dynabeads®/ml in phosphate buffered saline （PBS）, pH 7.4, with 0.1% bovine serum albumin （HSA）. Store at: 2-8°C.