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innovex biosciences(innovexbio)產(chǎn)品介紹
Fc Receptor Blocker – (4x Concentrate)

Fc 受體 (FcR) 是分子量約為 50-70 kD 的糖蛋白。他們被廣泛表達(dá)整個(gè)免益細(xì)胞。 Fc 受體存在于白細(xì)胞上，例如單核細(xì)胞、組織巨噬細(xì)胞、B 細(xì)胞、粒細(xì)胞（嗜酸性粒細(xì)胞、嗜堿性粒細(xì)胞、中性粒細(xì)胞）、NK 細(xì)胞和一些 T 細(xì)胞。 Fc受體也存在于肥大細(xì)胞上，濾泡樹(shù)突細(xì)胞、上皮細(xì)胞、內(nèi)皮細(xì)胞、肝細(xì)胞和朗格漢斯細(xì)胞等。有幾個(gè)

Fc 受體 (FcR) 的類型，根據(jù)它們識(shí)別的抗體進(jìn)行分類。有那些偉大的對(duì)單體 IgG 抗體的 Fc 區(qū)有親和力，稱為 Fc-Gamma 受體，與 IgA 抗體結(jié)合的稱為 Fc-Gamma 受體稱為 Fc-α 受體和結(jié)合 IgE 抗體的那些稱為 Fc-ε 受體。

IHC、ICC、免疫熒光 (IF) 和流式細(xì)胞術(shù)等檢測(cè)中的非特異性 Fc 受體染色可能是由于一抗和/或二抗或免疫球蛋白同種型的 Fc 區(qū)與抗體 Fc 受體的結(jié)合細(xì)胞。消除 Fc 受體染色在 IHC、ICC、IF 和流式細(xì)胞術(shù)檢測(cè)中是可取的，目的是為了準(zhǔn)確

數(shù)據(jù)評(píng)估。

For IHC, ICC, IF testing: The binding of the Fc region of primary and/or secondary antibody and immunoglobulin

isotypes to the Fc receptors present on cells/tissues give rise to non-specific Fc receptor staining. Specimens rich in Fc

receptors include spleen, lymphoid tissues, lymphomas, tonsil, lymph nodes, bone marrow, blood smears and liver. Others

include tissues and cell preps intended for staining for CD markers; Immunoglobulins (Igs), Kappa and Lambda and tumor

cell lines that express Fc receptors.

To avoid Fc receptor staining; tissue sections and cell preparations are incubated with Innovex Fc Blocker for a short period

prior to application of primary antibody or immunoglobulin isotypes (See instruction section of this data sheet).

For Flow cytometry assays: Fc receptors are present on leukocytes (white blood cells), many cell lines as well as other

cell types in both human and animal cells/tissues. Fc receptors gives rise to non-specific Fc receptor-staining in flow

cytometry assays by binding of Fc region of antibodies and isotype controls to Fc receptors of the cells. Non-specific Fc

receptors staining can be eliminated by incubating cells with INNOVEX Fc Receptor Blocker for a variety of Flow cytometry

assays such as CD phenotyping, leukemia typing and cell line studies. (See instruction section of this data sheet).

PRODUCT DESCRIPTION

Innovex Fc Receptor Blocker is a UNIVERSAL Fc Blocker applicable to blocking all types of Fc receptors such as Fc-gamma receptors of type I,

II and III; Fc -epsilon receptors type I and II; Fc –alpha receptors, Fcα/ µR and FcRn.

Innovex Fc Blocker does NOT contain antibodies, Immunoglobulins or immunoglobulin fragments.

INNOVEX Fc Blocker can be universally applied to block all types of Fc receptors in all-species including human, mouse

and all-animals This Universal Fc Block can be used in a variety of Immunoassays such as IHC, ICC, Immunofluorescence

and Flow cytometry. INNOVEX UNIVERSAL Fc BLOCK is suitable for blocking Fc receptors in frozen and paraffin tissue

sections and cyto preps of human and all-animal species.

INNOVEX Fc Blocker is also commonly used for eliminating background staining in brain cells/tissues.