三级片视频播放,精品三级片在线观看,A级性爱视频,欧美+日韩+国产+无码+小说,亲子伦XX XX熟女,秋霞最新午夜伦伦A片黑狐,韩国理伦片漂亮的保拇,一边吃奶一边做边爱完整版,欧美放荡性护士videos

Organism:

Homo sapiens, human

Tissue:

lung

Culture Properties:

adherent

Morphology:

epithelial

Complete Growth Medium:

RPMI-1640 (Gibco 31800-022)+10% FBS+ Penicillin/Streptomycin

Subculturing:

Volumes are given for a 25cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

• Remove and discard culture medium.
• Briefly rinse the cell layer with 0.25%(w/v) Trypsin-EDTA solution to remove all traces of serum that contains trypsin inhibitor.
• Add 1.0 to 2.0mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Discard Trypsin-EDTA solution.
• Add 6.0 to 8.0mL of complete growth medium and aspirate cells by gently pipetting.
• Add appropriate aliquots of the cell suspension to new culture vessels.

Cultures can be established between 2×10³ and 1×10⁴ viable cells/cm². Do not exceed 7×10⁴ cells/cm².

• Incubate cultures at 37°C, 5% CO₂.

Subc*tion Ratio:

A subc*tion ratio of 1:3 to 1:8 is recommended

Cryopreservation:

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase