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92283ESRecombinant Human R spondin 3/RSPO3 Protein,His Ta
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Human R spondin 3/RSPO3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln22-Val146.[Accession | Q9BXY4-1]
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規(guī)格

Recombinant Human R spondin 3/RSPO3 Protein,His Tag

92283ES60

100 μg

92283ES76

500 μg

 

性能參數(shù)

表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)

Human R spondin 3/RSPO3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln22-Val146.[Accession | Q9BXY4-1]

分子量大?。?/span>Molecular Weight)

The protein has a predicted MW of 14.95 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.

內(nèi)毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

純度(Purity)

> 95% as determined by Tris-Bis PAGE

 

 

活性(Activity)

ELISA DataImmobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA 

SPR DataHuman LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200)

制劑(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

儲存條件

The product should be stored at -25~-15℃ for 1 year from date of receipt.  2-7 days, 2 ~8 °C under sterile conditions after reconstitution.  3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事項

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.          

 




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