Gus基因染色*,In order to identify transformed cells or plants that have been growing on a selective medium, it is necessary to have an easily assayable reporter gene.
X-gluc描述:
Gus基因染色*,In order to identify transformed cells or plants that have been growing on a selective medium, it is necessary to have an easily assayable reporter gene. （X-glu, X-glcA） 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronide cyclohexylammonium salt
Sold as 1 g units
MOLECULAR BIOLOGY GRADE:C14 H13 Br Cl N O7? C6 H13 N
M.W.:521.79
Purity: greater than 99% （HPLC）
Thin Layer Chromatogram: single spot
Biological Activity: indigo-blue assay
Elemental Analysis: Agrees with theoretical ＆#118alues
CAS RN: [114162-64-0 ]
操作步驟
1. Add 200μl of acetone, vortex briefly.
2. Remove acetone and pipette into flat-bottom 96-well plate （polystyrene）
3. Centrifuge plate at 3000 x g for 3-5 minutes.
4. Invert plate carefully and blot dry with paper towels
5. Add 40μl of X-Gluc stain solution to each well （multi-pipettor） and incubate at
37°C overnight.
溶液配制
1 20 mg/ml X-Gluc concentration. e.g. For a 1.5 ml tube （microfuge tube）
dissolve 30 mg （.03 g） of X-Gluc in 1.5 ml DMSO （dimethyl sulfoxide）.
2 Cover with foil.
3 Vortex well.
4 Keep at -20°C.
解決方法
*Note: 4ml of stain solution are necessary to stain 1 96-well plate.
相關論文
1. Ourmanov, I. et al. （2009）. Improved Survival in Rhesus Macaques Immunized with Modified Vaccinia Virus. Journal of Virology, 83（11）, 5388-5400.
2. Coque, L. et al. （2008）. Transcription of ENOD8 in Medicago truncatula Nodules Directs ENOD8 Esterase to Developing and Mature Symbiosomes. Molecular Plant-Microbe Interactions, 21（4）, 404-410.
3. Kim, H., & Woloshuk, C.P. （2008）. Role of AREA, a regulator of nitrogen metabolism, during colonization of maize kernels and fumonisin biosynthesis in Fusarium verticillioides. Fungal Genetics and Biology, 45 （6）, 947-953.
4. Scarpeci, T.E. （2008）. Generation of superoxide anion in chloroplasts of Arabidopsis thaliana during active photosynthesis: a focus on rapidly induced genes . Plant Molecular Biology, 66（4）, 361-378.
5. Geddie, M.L., & Matsumura, I. （2007）. Antibody-Induced Oligomerization and Activation of an Engineered Reporter Enzyme. Journal of Molecular Biology, 369 （4）, 1052-1059.
6. Ko et al. （2004）. In Vitro Cellular and Developmental Biology- Plant, 40 （6）. 552-558.
7. Curtis et al. （2000）. Annals of Botany, 86. 63-71.
8. Ingram et al. （2000）.Plant Cell, Tissue and Organ Culture, 57. 207-210.
訂貨信息
| 貨號 | 名稱 | 產(chǎn)地 | 規(guī)格 | *（元） | 庫存 |
| G1281C | x-gluc GUS基因染色 | Goldbio | 100mg | 480 | 是 |
| G1281C1 | x-gluc GUS基因染色 | Goldbio | 1g | 1800 | 是 |
初級會員·13年
