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NUCLEOSIL® C18 HD

Octadecyl modified High Density silica phase for HPLC

Monomeric modification, endcapped · USP L1

Features

Hydrophobic, nonpolar phase for RP chromatography
Pore size 100 Å, available particle sizes 3 µm and 5 µm
pH stability 2–9, carbon content 20 %
Suited for separation of basic compounds such as pharmaceuticals
Separation mechanism based on hydrophobic (van der Waals) interactions
Corresponding NUCLEODUR® phase: see NUCLEODUR® C18 Gravity

For numerous applications click here:

使用LC-MS對磺胺類藥物的檢測

色譜柱：Nucleosil 100-5 C18 HD 帶保護柱

流動相：A)1mM醋酸胺溶液，醋酸調節PH到4.6+10%乙腈  B)*乙腈 梯度洗脫

流 速：0.25毫升/分鐘

進樣量：50ul

溫 度：25℃

檢測器：MS

樣 品：1. sulfaguanidine   2. sulfadiazine  3. sulfathiazole  4. N4-acetyl-sulfaguanidine  5. trimethoprim  6. sulfamethazine
7.sulfamethoxazole   8. sulfadimethoxine

作 者：Haller.M.Y. 等Joumal of Chromatography A952 (2002)111-120

 空氣中DNPH衍生醛酮類樣品

色譜柱：EC 250×3mm Nucleosil 100-5 HD C18

前處理：SPE on CHROMABOND DNPH—固相萃取 302900

流動相：EPA TO11 方法分離17種1 ng DNHP衍生醛酮類樣品

                A:水 B:乙腈 梯度洗脫：50% B to * B in 37.5 min.

流 速：560微升/分鐘

檢測器：Finnigan LCQ multiple stage MS.APCI-mode(negative ion detection)

樣 品：1. 甲醛  2. 乙醛  3. 丙酮  4. 丙醛  5. 2-ding烯醛  6. 異丁烯醛  7. 丁酮   8. 正丁醛  9. 苯甲醛  10. 異戊醛  11. 戊醛  12. O-甲基苯甲醛  13. M-甲基苯甲醛  14.P-甲基苯甲醛  15. 正己醛  16. 2,5-甲基苯甲醛

 紡織品和皮革中“芳香染料”的檢測

色譜柱：EC 250×4.6mm Nucleosil 100-5 C18 HD

前處理：XTR 萃取柱(302100方法)

流動相：A)NH4H2PO4 and Na2HPO4,0.005 mol each B)甲醇                梯度脫洗：10  62% B in 75 min

流 速：0.67毫升/分鐘

進樣量：10ul

溫 度：30℃

檢測器：DAD

樣 品：22種禁用偶氮染料

EC standard columns for HPLC

Ordering information

Eluent in column acetonitrile – water

Length

125 mm

150 mm

250 mm

EC guard columns*

CC guard columns**

NUCLEOSIL® 100-3 C18 HD · 3 µm particle size

4 mm ID

720191.40

721196.30

721494.40

4.6 mm ID

720191.46

720193.46

721196.30

721494.40

NUCLEOSIL® 100-5 C18 HD · 5 µm particle size

4 mm ID

720296.40

720280.40

721072.30

721853.40

4.6 mm ID

720296.46

720294.46

720280.46

721072.30

721853.40

Columns/pack

1

1

1

3

3

* EC 4/3 guard column cartridges are used for EC columns of 4 and 4.6 mm ID with the Column Protection System guard column holder (REF 718966).
** CC 8/4 guard column cartridges are used for EC columns of 4 and 4.6 mm ID with guard column adapter EC (REF 721359).

Custom-packed EC columns with other dimensions are available on request.