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NUCLEOGEN® DEAE

Silica-based HPLC phases for anion exchange chromatography of nucleic acids

Features

Base material silica, particle size 7 µm
DEAE anion exchanger
Eluent in column methanol

NUCLEOGEN® 60-7 DEAE: pore size 60 Å
for separation of oligonucleotides up to chain lengths of 40 bases with recoveries > 95 %
capacity 200 A260/mL (~ 300 A260 for a 125 x 4 mm ID column, 1875 A260 for a 125 x 10 mm ID column)
preparative separations possible when using higher flow rates and longer gradient times

NUCLEOGEN® 500-7 DEAE: pore size 500 Å
for the separation of tRNA, 5S RNA, viroids and messenger RNA in the intermediate molecular weight range (25,000–1,000,000 daltons) with recoveries > 95 %
capacity 730 A260 for a 125 x 6 mm ID column, 1940 A260 for a 125 x 10 mm ID column
 

NUCLEOGEN® 4000-7 DEAE: pore size 4000 Å
for the separation of plasmids, DNA restriction fragments, ribosomal RNA, messenger RNA and viral RNA, i.e., very high molecular weight nucleic acids (e.g., 1–50 megadaltons)
capacity 120 A260 for a 125 x 6 mm ID column, 350 A260 for a 125 x 10 mm ID column
For more applications click here.

Ordering information

Length 125 mm

CC guard columns*

NUCLEOGEN® 60-7 DEAE

EC analytical columns

4 mm ID

736596.40

736400.40

VarioPrep preparative columns

10 mm ID

736597.100

736400.40

NUCLEOGEN® 500-7 DEAE

Valco type analytical columns

6 mm ID

736598

736400.40

VarioPep preparative columns

10 mm ID

736599.100

736400.40

NUCLEOGEN® 4000-7 DEAE

Valco type analytical columns

6 mm ID

736601

736400.40

VarioPrep preparative columns

10 mm ID

736602.100

736400.40

* ChromCart® NUCLEOGEN® guard column cartridges measure 30 x 4 mm, require the CC column holder 30 mm (REF 721823) and are supplied in packs of 2 (all other columns in packs of 1).