CBP73298 | |
| I. Introduction | |
Cell Line Name： | c-Kit L576P/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
KIT L576P lies within the juxtamembrane domain (exon 11) of the Kit protein (PMID: 17372901). L576P confers a gain of function to Kit, as indicated by constitutive phosphorylation of Kit, and is transforming in cell culture (PMID: 17372901). | |
| III. Representative Data | |
1. WB of c-Kit L576P/BaF3
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2. Sanger of c-Kit L576P/BaF3
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3. Anti-proliferation assay | |
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Figure 3. CTG Proliferation Assay of BaF3 c-KIT L576P (C6). | |
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