| I. Background | |
| CD40 是腫瘤壞死因子受體超家族（TNFRSF）成員，作為免疫調(diào)節(jié)的關(guān)鍵分子，廣泛 參與 B 細(xì)胞活化、抗原呈遞細(xì)胞（APC）成熟及 T 細(xì)胞免疫應(yīng)答。其配體 CD40L（CD154） 主要表達(dá)于活化的 T 細(xì)胞，通過 CD40-CD40L 互作橋接先天性與適應(yīng)性免疫，是治療、自身免疫病及感染性疾病的熱門靶點(diǎn)。 CD40 信號(hào)轉(zhuǎn)導(dǎo)主要利用 TNF 受體相關(guān)因子的銜接蛋白，導(dǎo)致經(jīng)典和非經(jīng)典 NFκB 通 路、MAPK、PI3K 和 PLCγ的激活。它們的激活導(dǎo)致特征性下游效應(yīng)，包括轉(zhuǎn)錄激活、細(xì) 胞骨架重排和細(xì)胞存活。 | |
| II. Description | |
CD40 NFκB-Luc HEK293 報(bào)告基因藥靶模型很好的模擬了體內(nèi) CD40 的信號(hào)轉(zhuǎn)導(dǎo)過程， 原理見下圖所示。
Figure 1. CD40 NFκB-Luc HEK293 細(xì)胞模型原理圖 | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | CD40 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-κB cell line, NFκB cell line, CD40 cell line, cd40 luciferase reporter, CD40 HEK293 cell line |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM+10%FBS+1% NEAA+1 mM NaP+1 μg/ml Puromycin+100 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | kidney |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
Figure 2. Recombinant HEK293 cell line expressing firefly luciferase gene under the control of NFκB response elements with constitutive expression of CD40. | |
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