LAG3/IL2-Luc/Jurk
| CBP74079 | |
| I. Background | |
Lymphocyte-activation gene 3 (LAG3, CD223) is a cell surface protein that belongs to immunoglobulin (Ig) superfamily. LAG3 is expressed on activated T cells, natural killer cells, B cells, and plasmacytoid dendritic cells. | |
| II. Description | |
| Recombinant Jurkat T cell expressing firefly luciferase gene under the control of IL-2 response elements with constitutive expression of human LAG3 (lymphocyte-activation gene 3, CD223, GenBank Accession # NM_002286). | |
| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: | LAG3-IL2-Luc |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | CD223, LAG-3, Ly66, lymphocyte activating 3, lymphocyte-activation gene 3, LAG3, Lag3, IL-2 Promoter reporter, lentivirus reporter, HIV reporter, VSV-G reporter |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1ug/ml puromycin+10ug/ml blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Peripheral blood |
| Disease: | Childhood T acute lymphoblastic leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
| |
Figure 1. Recombinant Jurkat stably expressing human LAG3. | |
Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose Response of Anti-LAG3 Neutralizing Antibody in LAG3/lL2 Promoter Reporter-Jurkat Cells, the EC50=2.99ug/ml. |
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