| CBP73378 | |
| I. Introduction | |
Cell Line Name： | PDGFRA V658A-V561A/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
PDGFRA, platelet-derived growth factor receptor alpha, is a receptor tyrosine kinase that activates PI3K/Akt, RAS/MAPK, and JAK/STAT pathways to promote cell proliferation and survival (PMID: 21605429). PDGFRA mutations have been detected in melanoma, AML, peripheral nerve sheath tumors, sarcoma, and neuroendocrine carcinomas (PMID: 23752188, PMID: 20685895), several fusions have also been reported (PMID: 22432087, PMID: 26276769), and amplification has been observed in glioblastoma (PMID: 23438035). | |
| III. Representative Data | |
1.Sanger of PDGFRA V658A-V561A/BaF3
Figure 1. PDGFRA V658A-V561A/BaF3 V658A
Figure 2. PDGFRA V658A-V561A/BaF3 V561A | |
2. Anti-proliferation assay
Figure 3. CTG Proliferation Assay of BaF3 PDGFRA V658A Cells (C3). | |
![CBP73378 sanger-V658A V658A]BaF3-SFFV-PDGFRA-V658A-V561A-C3.jpg](https://img69.chem17.com/33c3386a40defc478ef3be6d8fab0d19b9180d3c36d69528adc7b2553714f549194528a68e379c7d.jpg "CBP73378 sanger-V658A V658A]BaF3-SFFV-PDGFRA-V658A-V561A-C3.jpg")
![CBP73378 sanger-V561A [V561A]BaF3-SFFV-PDGFRA-V658A-V561A-C3.jpg](https://img63.chem17.com/33c3386a40defc478ef3be6d8fab0d1969b1d978f7ef7970a8e810066f78921cd16130bfb0bae3e2.jpg "CBP73378 sanger-V561A [V561A]BaF3-SFFV-PDGFRA-V658A-V561A-C3.jpg")
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