hCD166/4T1
| I. Background | |||
| CD166（也稱(chēng)為 ALCAM）是一種跨膜糖蛋白，屬于免疫球蛋白超家族（IgSF）成員。 其在多種腫瘤中高表達(dá)，包括結(jié)直腸癌、乳腺癌、前列腺癌和黑色素瘤；并在多種細(xì)胞類(lèi) 型中表達(dá)，包括上皮細(xì)胞、內(nèi)皮細(xì)胞、神經(jīng)元和免疫細(xì)胞。CD166 在細(xì)胞間相互作用、信 號(hào)傳導(dǎo)和細(xì)胞遷移中起重要作用，同時(shí)也與腫瘤的發(fā)生、發(fā)展和轉(zhuǎn)移密切相關(guān)。CD166 通 過(guò)其胞外區(qū)與其他細(xì)胞表面分子（如 CD166 或 CD6）結(jié)合，介導(dǎo)細(xì)胞間粘附；還可以通過(guò) 激活下游信號(hào)通路（如 PI3K/AKT 或 MAPK 通路）調(diào)節(jié)細(xì)胞增殖、存活和遷移。 | |||
| II. Introduction | |||
| Host Cell: | 4T1 | ||
| Expressed gene: | hCD166 | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | RPMI-1640+10%FBS+ 2 μg/ml Puromycin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mouse | ||
| Tissue: | Mammary gland | ||
| Morphology: | Epithelial | ||
| Growth Properties: | Adherent | ||
| IV. Representative Data
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Figure 1. Recombinant hCD166/4T1 stably expressing hCD166. | |||
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