Recombinant Phosphatidic Acid Phosphatase Type 2B (PPAP2B)

磷脂酸磷酸酶2B(PPAP2B)重組蛋白

[ PROPERTIES ]

Residues:Ser57~Ser194

Tags:Two N-terminal Tags, His-tag and T7-tag

Accession:O14495

Host: E. coli

Subcellular Location:Golgi Apparatus;Membrane; multi-pass Membrane Protein.

Purity: >95%

Endotoxin Level:<1.0EU per 1μg(determined by the LAL method).

Formulation:Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point:9.0

Predicted Molecular Mass: 19.3kDa

Applications:SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH₂O..

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8oC for one month.

           Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The sequence of the target protein is listed below.

STIK PYHRGFYCND ESIKYPLKTG ETINDAVLCA VGIVIAILAI ITGEFYRIYY LKKSRSTIQNPYVAALYKQV GCFLFGCAIS QSFTDIAKVS IGRLRPHFLS VCNPDFSQIN CSEGYIQNYRCRGDDSKVQE ARKS

[  IDENTIFICATION  ]

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