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貨物所在地：北京北京市
產(chǎn)地：FireGen
更新時間：2025-07-16 09:47:37
有效期：2025年7月16日 -- 2026年1月13日
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(SARS-CoV-2)核酸檢測試劑盒(實時熒光探針法)
Novel Coronavirus (SARS-CoV-2) Nucleic Acid Detection Kit (Real-Time RT-PCR Method)
FiregenSARS-CoV-2?核酸檢測試劑盒
產(chǎn)品優(yōu)勢：全組份凍干技術(shù)，常溫運輸，無需冷鏈，比液體試劑更可靠，創(chuàng)新采用單反應(yīng)微球設(shè)計，簡化操作流程
Novel Coronavirus (SARS-CoV-2) Nucleic Acid Detection Kit (Real Time RT-PCR Method)
Instructions for Use
Intended Use
The FiregenTM Novel Coronavirus (SARS-CoV-2) Nucleic Acid Detection Kit is a real-time multiplex RT-PCR test intended for the qualitative detection of SARS-CoV-2 viral RNA. It is suitable for upper respiratory specimens, such as nasopharyngeal, oropharyngeal swab, and bronchoalveolar lavage specimens. In a single RT-PCR reaction, it is able to detect ORF1ab and N RNA sequences, and in addition, human RNase P as an internal control.
Principles
This kit uses the real-time fluorescent RT-PCR technology, with prime and probe sets designed targeting the ORF1ab and N gene sequences. Each probe is labeled at the 5 ' end a fluorescence reporter group and 3' end a quenching group. The 3’ primers enable the reverse transcriptase to generate cDNA from viral RNA, and subsequently Taq polymerase performs multiple rounds of amplification of the target DNA. During primer extension steps, the 5 '→ 3' exonuclease activity of the Taq enzyme hydrolyzes the probes, and the reporter fluorescence group is free from the quencher, and therefore a fluorescent signal is emitted. The target detection can be achieved from the amplification curve and the Ct value.
This kit contains an endogenous internal control (IC), which targets human RNase P gene sequences. The IC is used to monitor the collection, transportation, extraction and amplification of the test specimen to avoid false negativity.
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