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當前位置：> 供求商機> 410101-50mL——環氧活化瓊脂糖凝膠4HF
Product Introduction:
Epoxy-activated agarose beads can be used for the immobilization of sugars and other carbohydrates via stable ether linkages with hydroxyl groups. They can also be used for the coupling of primary amine-containing, or thiol-containing molecules.
Coupling biospecific ligands to Epoxy-activated agarose is a successful and well-documented technique. The reaction is very easy to conduct. No toxic chemical or special equipment is required. All the chemical bonds formed are very stable.
Epoxy activated SepFast is useful in coupling both small molecules and very large molecules. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography media for both small scale and large scale purification applications.
Product Properties:
There is a choice of two different Epoxy activated base matrices that suits various ligands and applications.
Epoxy activated SepFast 4HF is made of highly cross-linked 4% beaded agarose. It shows good mechanical rigidity allowing high flow throughput with reduced back pressure. It is useful for the coupling of protein molecules.
Epoxy activated SepFast 6HF is made of highly cross-linked 6% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure. It has high levels of epoxy groups for applications that require high ligand densities and high flow properties. It is useful for the coupling of small molecules or peptides etc.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.
The material is activated through attaching a hydrophilic carbon chain with epoxy group in the end.
Group to be coupled | -NH2, -OH, -SH |
Matrix | SepFast 4HF: Highly cross-linked 4% agarose bead SepFast 6HF: Highly cross-linked 6% agarose bead |
Particle size | 50 – 150 µm |
Activation level | > 30 µmol epoxy / ml for SepFast 4HF >80 µmol epoxy / ml for SepFast 6HF |
pH stability | 2 -13 (ligand dependent) |
Chemical stability | Compatible with all commonly used aqueous chemicals, provided the ligand to be coupled can withstand. Certain organic solvents could be used. |
Storage | +4℃—+8℃ |
Product Series：
| Product | Quantity | Code no. |
| Epoxy activated SepFast 4HF | 50 ml | 410101-50ML |
| 300 ml | 410101-300ML | |
| 1 litre | 410101-1L | |
| Epoxy activated SepFast 6HF | 50 ml | 410104-50ML |
| 300 ml | 410104-300ML | |
| 1 litre | 410104-1L |
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