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湘潭大學城市污水中的季銨鹽對綠藻NH4+吸收的影響
期刊：Science of The Total Environment
主題：城市污水中的季銨鹽對綠藻NH4+吸收的影響
標題：Extracellular polymeric substrates of Chlorella vulgaris F1068 weaken stress of cetyltrimethyl ammonium chloride on ammonium uptake
影響因子：4.610
監(jiān)測指標：NH4+流速
檢測部位：小球藻藻細胞
NH4+流速流實驗處理方法：
小球藻，有無0.5 mg/L CTAC在光照/黑暗下處理6小時
NH4+流速流實驗測試液成份：
NH4Cl 37.4 mg/L, NaH2PO42·7H2O 9.8 mg/L, CaCl2·2H2O 14.7 mg/L,MgSO4·7H2O 24.6 mg/L and NaHCO3 42mg/L，pH 7.5
作者：湘潭大學葛飛、匡揚鐸
英文摘要
This study investigated the influences of cetyltrimethyl trimethyl ammonium chloride (CTAC), an emerging pollutant quaternary ammonium compound (QAC) in municipal effluents, on the transfer and uptake of NH4+ by Chlorella vulgaris F1068 cells removed EPS artificially (EPS-R) and coated EPS naturally (EPS-C) under different scenarios (e.g., the presence or absence of CTAC, different photoperiod sequences (light 12?h: dark 12?h or dark 12?h: light 12?h)).
The results showed that the removal of EPS increased the transfer and uptake of NH4+ but the presence of EPS caged NH4+ and effectively weakened the stress of CTAC (<0.5?mg/L) on NH4+ uptake. The main mechanism was considered that CTAC in the concentration range from 0.1 to 0.5?mg/L induced an increased amount of polysaccharide and protein in EPS and thus protected algal normal physiological functions (including cell membrane permeability and glutamine synthetase activity) from the damage of CTAC (0.1 to 0.5?mg/L) regardless of the photoperiod sequences.
Thereby, the findings of this study provided an insight into the role of algal EPS in transfer and uptake of nutrients under the coexisted toxics for the future algae-based sewage treatment application.
中文摘要（谷歌機翻）
這項研究調(diào)查了十六烷基*基*基氯化銨（CTAC）（一種新興的污染物季銨化合物（QAC））在城市污水中對尋常綠藻F1068細胞人工去除EPS（EPS-R）和包被EPS的轉(zhuǎn)移和吸收NH4+的影響。自然地（EPS-C）在不同情況下（例如，是否存在CTAC，不同的光周期序列（亮12 h：暗12 h或暗12 h：亮12 h））。
結(jié)果表明，EPS的去除增加了NH4+的轉(zhuǎn)移和吸收，但是EPS的存在籠罩了NH4+，有效地減弱了CTAC（<0.5μmg/ L）對NH4+吸收的脅迫。認為其主要機理是濃度在0.1至0.5μmg/ L的CTAC誘導EPS中的多糖和蛋白質(zhì)增加，從而保護藻類的正常生理功能（包括細胞膜通透性和谷氨酰胺合成酶活性）免受CTAC的損害。 （0.1至0.5μmg/ L），與光周期無關(guān)。
因此，本研究的發(fā)現(xiàn)為藻類EPS在未來基于藻類的污水處理應用中在共存有毒物質(zhì)的轉(zhuǎn)移和吸收養(yǎng)分中的作用提供了見解。
結(jié)果表明：在沒有CTAC的6小時光照下，EPS-C細胞或EPS-R細胞的NH4+通量分別達到-1.87ng /（cm2·s）和-2.63ng /（cm2·s）。當添加0.5 mg / L的CTAC時，EPS-C或EPS-R細胞的NH4+通量急劇下降至-0.94 ng /（cm2·s）和-0.73 ng /（cm2·s）。相反，當EPS-R細胞和EPS-C細胞在黑暗中培養(yǎng)6小時無論CTAC存在與否，EPS-C細胞和EPS-R細胞的NH4+流速從負值轉(zhuǎn)換為正值，表明NH4+從細胞內(nèi)環(huán)境流出到培養(yǎng)基。