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轉基因擬南芥中過表達景天苜蓿Cu/Zn超氧化物歧化酶提高對氧化應激抗性
NMT作為生命科學底層核心技術，是建立活體創(chuàng)新科研平臺的*技術。2005年~2020年，NMT已扎根中國15年。2020年，中國NMT銷往瑞士蘇黎世大學，正式打開歐洲市場。
NMT歷*的今天
2017年06月13日，中國林科院亞熱帶林業(yè)研究所卓仁英用NMT發(fā)表了標題為Overexpressing the Sedum alfredii CuZn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis的研究成果。
期刊：Frontiers in Plant Science
主題：在轉基因擬南芥中過表達景天苜蓿Cu/Zn超氧化物歧化酶提高了對氧化應激的抗性
標題：Overexpressing the Sedum alfredii CuZn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis
檢測指標：Cd2+流速
作者：中國林科院亞熱帶林業(yè)研究所卓仁英、韓小嬌、李真
英文摘要
Superoxide dismutase (SOD) is a very important reactive oxygen species (ROS)- scavenging enzyme. In this study, the functions of a Cu/Zn SOD gene (SaCu/Zn SOD), from Sedum alfredii, a cadmium (Cd)/zinc/lead co-hyperaccumulator of the Crassulaceae, was characterized.
The expression of SaCu/Zn SOD was induced by Cd stress. Compared with wild-type (WT) plants, overexpression of SaCu/Zn SOD gene in transgenic Arabidopsis plants enhanced the antioxidative defense capacity, including SOD and peroxidase activities.
Additionally, it reduced the damage associated with the overproduction of hydrogen peroxide (H2O2) and superoxide radicals (O2 ). The influence of Cd stress on ion flux across the root surface showed that overexpressing SaCu/Zn SOD in transgenic Arabidopsis plants has greater Cd uptake capacity existed in roots. A co-expression network based on microarray data showed possible oxidative regulation in Arabidopsis after Cd-induced oxidative stress, suggesting that SaCu/Zn SOD may participate in this network and enhance ROS-scavenging capability under Cd stress.
Taken together, these results suggest that overexpressing SaCu/Zn SOD increased oxidative stress resistance in transgenic Arabidopsis and provide useful information for understanding the role of SaCu/Zn SOD in response to abiotic stress.
中文摘要（谷歌機翻）
超氧化物歧化酶（SOD）是一種非常重要的活性氧（ROS）清除酶。在這項研究中，表征了景天科植物景天科植物鎘（Cd）/鋅/鉛超積累的Cu / Zn SOD基因（SaCu / Zn SOD）的功能。
鎘脅迫誘導了SaCu / Zn SOD的表達。與野生型植物相比，轉基因擬南芥植物中SaCu / Zn SOD基因的過表達增強了其抗氧化防御能力，包括SOD和過氧化物酶活性。
此外，它還減少了與過氧化氫（H2O2）和超氧自由基（O2）過量產生相關的損害。Cd脅迫對根表面離子通量的影響表明，過表達SaCu / Zn SOD在轉基因擬南芥植物中具有更大的Cd吸收能力。基于微陣列數據的共表達網絡表明，Cd誘導的氧化脅迫后擬南芥中可能存在氧化調控，這表明SaCu / Zn SOD可能參與該網絡并增強Cd脅迫下的ROS清除能力。
綜上所述，這些結果表明，過表達SaCu / Zn SOD在轉基因擬南芥中提高了抗氧化脅迫的能力，并為理解SaCu / Zn SOD在響應非生物脅迫中的作用提供了有用的信息。
此外，暴露于Cd下的細胞壁厚度下降導致HE細胞壁中較少的細胞壁鍵合Cd。因此，Cd誘導的根部特征以及根尖的改變導致了兩種生態(tài)型苜蓿鏈球菌對Cd吸收和積累的差異。
FIGURE 4 | Net Cd2C fluxes. Net Cd2C fluxes in the roots of transgenic (OE2, OE3, and OE4) and WT plants treated without (A) or with CdCl2 stress (B), respectively. The average 300 s net Cd2C fluxes (C) are illustrated to highlight the trend differences. Bars indicate means SD. Asterisks indicate significant differences at p < 0.05 and p < 0.01.