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NMT歷*的今天丨硒調(diào)節(jié)木質(zhì)素合成及鎘相關(guān)基因表達(dá)促水稻拒鎘
NMT作為生命科學(xué)底層核心技術(shù)，是建立活體創(chuàng)新科研平臺的*技術(shù)。2005年~2020年，NMT已扎根中國15年。2020年，中國NMT銷往瑞士蘇黎世大學(xué)，正式打開歐洲市場。
NMT歷*的今天
2018年07月01日，廣東省科學(xué)院生態(tài)環(huán)境研究所李芳柏用NMT發(fā)表了標(biāo)題為Selenium reduces cadmium uptake into rice suspension cells by regulating the expression of lignin synthesis and cadmium-related genes的研究成果。
期刊：Science of The Total Environment
主題：硒通過調(diào)節(jié)木質(zhì)素合成和鎘相關(guān)基因的表達(dá)減少水稻對懸浮細(xì)胞的鎘吸收
標(biāo)題：Selenium reduces cadmium uptake into rice suspension cells by regulating the expression of lignin synthesis and cadmium-related genes
檢測指標(biāo)：Cd2+流速
作者：廣東省科學(xué)院生態(tài)環(huán)境研究所李芳柏、崔江虎
英文摘要
Although previous studies have indicated that selenium (Se) can reduce cadmium (Cd) uptake into rice, the mechanism at the cellular level has not been reported. Here, rice suspension cells exposed to Cd treatment in the presence or absence of Se were Compared with treatment with alone, pretreatment with Se increased the proportion of live cells by 83.1%. The levels of reactive oxygen species and mitochondrial membrane potential in the Se-pretreated rice cells were decreased by 86.6% and 76.0%, respectively.
In addition, non-invasive micro-test technology suggested that the mean values of Cd2+ influx decreased significantly in the Se-pretreated rice cells in a concentration-dependent manner. The results of inductively coupled plasmamass spectrometry (ICP-MS) showed that 67.4%–78.8% Cd accumulated onto the cell walls of the pretreated-Se rice cells. The addition of Se increased the lignin content and thickness of the cell walls, leading to an improved mechanical force of the cell walls, as determined by atomic force microscopy (AFM).
Furthermore, Se pretreatment decreased the expression of genes involved in Cd uptake (OsNramp5) and transport (OsLCT1) but activated the expression of genes involved in Cd transport into vacuoles (OsHMA3) and lignin synthesis (OsPAL, OsCoMT and Os4CL3). These results indicated that supplying Se alleviates Cd toxicity by regulating the express of lignin synthesis and Cd-related genes. The present findings provide new insights on a plausible explanation of the Se-reduced Cd uptake into rice.
中文摘要
盡管以前的研究表明硒可以減少水稻對鎘的吸收，但是在細(xì)胞水平上的機(jī)制尚未見報道。在此，將在有硒或無硒的情況下暴露于Cd處理的水稻懸浮細(xì)胞與單獨(dú)處理相比，用硒進(jìn)行預(yù)處理可使活細(xì)胞的比例增加83.1％。硒預(yù)處理的水稻細(xì)胞中的活性氧水平和線粒體膜電位分別降低了86.6％和76.0％。
此外，非損傷微測技術(shù)表明，硒預(yù)處理的水稻細(xì)胞中Cd2+流入量的平均值以濃度依賴的方式顯著降低。電感耦合等離子體質(zhì)譜法（ICP-MS）的結(jié)果表明，預(yù)處理的Se水稻細(xì)胞的細(xì)胞壁上積累了67.4％–78.8％的Cd。Se的添加增加了木質(zhì)素的含量和細(xì)胞壁的厚度，從而導(dǎo)致了細(xì)胞壁機(jī)械力的改善，這是通過原子力顯微鏡（AFM）確定的。
此外，Se預(yù)處理降低了Cd吸收（OsNramp5）和轉(zhuǎn)運(yùn)（OsLCT1）相關(guān)基因的表達(dá)，但激活了Cd轉(zhuǎn)運(yùn)至液泡（OsHMA3）和木質(zhì)素合成（OsPAL，OsCoMT和Os4CL3）的基因表達(dá)。這些結(jié)果表明提供硒通過調(diào)節(jié)木質(zhì)素合成和Cd相關(guān)基因的表達(dá)來減輕Cd毒性。目前的發(fā)現(xiàn)為對水稻中硒減少的鎘吸收的合理解釋提供了新的見解。
(A) Representative cell and the Cd2+-selective microelectrode; (B) The average Cd2+ flux values (mean ± SD) in the rice cells was detected in the absence or presence of Se.