Analyte Information

IFN-γ

Interferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

ELISABASIC kit for quantification of IFN-γ in solution

The human IFN-γ ELISABASIC kit is ideal for users who want the flexibility of setting up their own ELISA assay. This product enables quantification of human IFN-γ in solution, e.g. in cell culture supernatant. The kit contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-HRP, ELISA standard and standard reconstitution buffer. Plates and substrate are not included.

Human IFN-γ ELISABASIC kits are also available with Streptavidin-ALP.

Serum/Plasma samples

Analysis of serum/plasma samples with this kit requires the use of ELISA diluent for dilution of the samples, standard and detection antibody. The diluent prevents false positive read-outs which may be caused by interference of heterophilic antibodies found in serum and plasma. The ELISA diluent is available from Mabtech. See Related products.

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艾美捷人干擾素Y ELISA開發試劑盒，用于定量溶液中的 IFN-γ

人干擾素Y ELISA開發試劑盒非常適合希望靈活設置自己的 ELISA 檢測的用戶。該產品可以對溶液中的人 IFN-γ 進行定量，例如在細胞培養上清液中。該試劑盒包含一對匹配的單克隆捕獲和檢測抗體、Streptavidin-HRP、ELISA 標準和標準重組緩沖液。不包括板和基板。人干擾素Y ELISA開發試劑盒也可與 Streptavidin-ALP 一起使用。

血清/血漿樣本

使用艾美捷人干擾素Y ELISA開發試劑盒分析血清/血漿樣品，需要使用 ELISA 稀釋劑來稀釋樣品、標準品和檢測抗體。稀釋劑可防止由于血清和血漿中發現的異嗜性抗體的干擾而導致的假陽性讀數。ELISA稀釋劑可從Mabtech獲得。僅供研究使用 (RUO)。不用于診斷過程。

艾美捷人干擾素Y ELISA開發試劑盒標準曲線的繪制：

艾美捷人干擾素Y ELISA開發試劑盒內容：

捕獲 mAb (1-D1K)

生物素化檢測 mAb (7-B6-1)

鏈霉親和素-HRP

重組人 IFN-γ ELISA 標準品

標準重組緩沖液

應用：酶聯免疫吸附試驗

反應性：人類

標準范圍：4-400 皮克/毫升