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Aβ1-42猴β淀粉樣蛋白1-42酶聯(lián)免疫試劑盒操作步驟：

1. 編號(hào)：將樣品對(duì)應(yīng)微孔按序編號(hào)，每板應(yīng)設(shè)陰性對(duì)照 2 孔、陽(yáng)性對(duì)照 2 孔、空白對(duì)照 1孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑，其余各步操作相同）

2. 加樣：分別在陰、陽(yáng)性對(duì)照孔中加入陰性對(duì)照、陽(yáng)性對(duì)照 50%l。然后在待測(cè)樣品孔先加樣品稀釋液 40%l，然后再加待測(cè)樣品 10%l。加樣將樣品加 于酶標(biāo)板孔底部，盡量不觸及孔壁，輕輕晃動(dòng)混勻，

3. 溫育：用封板膜封板后置 37℃溫育 30 分鐘。

4. 配液：將 30 倍濃縮洗滌液用蒸餾水 30 倍稀釋后備用

5. 洗滌：小心揭掉封板膜，棄去液體，甩干，每孔加滿洗滌液，靜置 30 秒后棄去，如此重復(fù) 5 次，拍干。

6. 加酶：每孔加入酶標(biāo)試劑 50%l，空白孔除外。

7. 溫育：操作同 3。

8. 洗滌：操作同 5。

9. 顯色：每孔先加入顯色劑 A50%l，再加入顯色劑 B50%l，輕輕震蕩混勻，37℃避光顯色15 分鐘.

10. 終止：每孔加終止液 50%l，終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）。

11. 測(cè)定：以空白空調(diào)零，450nm 波長(zhǎng)依序測(cè)量各孔的吸光度（OD 值）。 測(cè)定應(yīng)在加終止液后 15 分鐘以內(nèi)進(jìn)行。

兔抗羊IgG (抗血清) Anti-Goat IgG antibody produced in rab... 1ML 免疫血清

兔抗豚鼠IgG (抗血清) Anti-Guinea Pig IgG antibody produced ... 1ML 免疫血清

羊抗馬IgG (抗血清) Anti-Horse IgG antibody produced in goat 1ML 免疫血清

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羊抗人白蛋白 (抗血清) Anti-Human Albumin antibody produced i... 1ML 免疫血清

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羊抗人C3 (抗血清) Anti-Human C3 antibody produced in goat 1ML 免疫血清

羊抗人纖維蛋白原 (抗血清) Anti-Human Fibrinogen produced in goat 1ML 免疫血清

兔抗人纖維蛋白原 (抗血清) Anti-Human Fibrinogen produced in rabbit 1ML 免疫血清

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羊抗人IgA (抗血清) Anti-Human IgA antibody produced in goat 1ML 免疫血清

兔抗人IgA (抗血清) Anti-Human IgA antibody produced in ra... 1ML 免疫血清

兔抗人IgG～Fc (抗血清) Anti-Human IgG (Fc spec.) produced in ... 1ML 免疫血清

羊抗人IgG (抗血清) Anti-Human IgG antibody produced in goat 1ML 免疫血清

兔抗人IgG (抗血清) Anti-Human IgG antibody produced in ra... 1ML 免疫血清

羊抗人IgM血清 (μ鏈特異) Anti-Human IgM (μ-chain spec.) produc... 1ML 免疫血清

羊抗人IgM (抗血清) Anti-Human IgM antibody produced in goat 1ML 免疫血清

兔抗人IgM (抗血清) Anti-Human IgM antibody produced in ra... 1ML 免疫血清

羊抗人血清 (多價(jià)抗體) Anti-Human Serum antibody produced in ... 1ML 免疫血清

兔抗人血清 (多價(jià)抗體) Anti-Human Serum antibody produced in ... 1ML 免疫血清

兔抗猴IgG (抗血清) Anti-Monkey IgG antibody produced in r... 1ML 免疫血清

兔抗小鼠IgG～Fc (抗血清) Anti-Mouse IgG (Fc spec.) produced in ... 1ML 免疫血清

羊抗小鼠IgG (抗血清) Anti-Mouse IgG antibody produced in goat 1ML 免疫血清

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兔抗HRP (抗血清) Anti-Peroxidase antibody produced in r... 1ML 免疫血清

羊抗豬IgG (抗血清) Anti-Pig IgG antibody produced in goat 1ML 免疫血清

羊抗兔IgG (抗血清) Anti-Rabbit IgG antibody produced in g... 1ML 免疫血清

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