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Source

Mouse

Applications

IF,WB,IHC

Reactivity

Human,Mouse,Rat,Chicken

Dilution

IF: 1:50-200 WB: 1:500-1000 IHC: 1:100-200

Storage

-20°C/1 year

Specificity

The antibody detects endogenous Active Caspase-3 protein.

Source/Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Immunogen

Recombinant Protein of Active Caspase-3

Uniprot No

P42574

Alternative names

CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

Form

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Clonality

Monoclonal

Isotype

IgG

Background

caspase 3(CASP3) Homo sapiens This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008],

Other

CASP3, Caspase-3; Protein Yama; SREBP cleavage activity 1; SCA-1

Concentration

1 mg/ml