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進口安捷倫Agilent Bio IEX色譜柱*
安捷倫生物離子交換 HPLC 柱填充了聚合物型的非多孔離子交換填料。 生物離子交換色譜柱是專為實現肽類、寡核苷酸和蛋白質的高分離度、高回收率和高效分離而設計的。
Bio IEX 系列色譜柱提供了強陽離子交換 (SCX)、弱陽離子交換 (WCX)、強陰離子交換(SAX) 和弱陰離子交換 (WAX) 填料。所有生物離子交換固定相均有粒徑 1.7、3、5 和 10 μm 的非多孔填料可供選擇。
- 高度交聯的非多孔聚苯乙烯二乙烯基苯 (PS/DVB) 硬質填料表面結合親水聚合物層，避免了非特異性鍵合
- 均勻、致密填裝的離子交換官能團化學鍵合到親水層上（每個位點連接多個離子交換基團），提高了色譜柱容量
- 填料、涂層和鍵合能夠耐高壓，有助于實現更高的分離度和更快速的分離。
- 多個離子交換基團固定連接在一個結合位點上，以增加柱容量。
| 色譜柱固定相 | SCX（強陽離子交換） — SO3H WCX（弱陽離子交換） — COOH SAX（強陰離子交換） — N(CH3)3 WAX（弱陰離子交換） — N(C2H5)2 | |||
| 裝填 | 非多孔、聚（苯乙烯二乙烯苯） (PS/DVB)，帶疏水涂層并鍵合有均勻的陽離子交換層 | |||
| 粒徑 | 1.7、3、5 和 10 μm | |||
| 孔隙結構 | 非多孔 | |||
| pH 穩定性 | 2-12 | |||
| 操作溫度上限 | 80 ºC | |||
| 柱硬件操作壓力上限 | 600 bar (8700 psi) 適用于不銹鋼柱硬件 400 bar (5800 psi) 適用于 EK 柱硬件 | |||
| 填料操作壓力上限 | 10 μm 填料 275 bar (4000 psi) 5 μm 填料 413 bar （6000 psi) 3 μm 填料 551 bar （8000 psi) 1.7 μm 填料 689 bar （10000 psi) | |||
| 流動相兼容性 | 與含水緩沖液、乙腈/丙酮/甲醇和水混合液兼容。常用緩沖液:磷酸鹽、tris、MES和醋酸鹽 | |||
| 工作流速 | 4.6 mm 內徑柱或 2.1 mm 內徑柱一般流速為 0.1-1.0 mL/min：從低流速開始，并將硬件和/或填料壓力上限中的較低值設置為默認值。 | |||
| 動態結合容量 | NP3 | NP5 | NP10 | |
| SCX | 53 mg/mL | 38 mg/mL | 20 mg/mL | |
| WCX | 19 mg/mL | 15 mg/mL | 10 mg/mL | |
| SAX | 35 mg/mL | 28 mg/mL | 17 mg/mL | |
| WAX | 26 mg/mL | 18 mg/mL | 12 mg/mL | |
進口安捷倫Agilent Bio IEX色譜柱*
以下為部分安捷倫同系列產品貨號清單，如果沒有找到合適的，歡迎隨時聯系 我們提供貨號獲取報價。
| 部件號 | ID (mm) | Length (mm) | Particle Size (µm) | Phase | Material | 單位 |
| 5190-2479 | 2.1 | 250 mm | 10 µm | Agilent Bio SAX | EK | |
| 5190-2472 | 2.1 | 50 mm | 5 µm | Agilent Bio SAX | EK | 1/pk |
| 5190-2471 | 2.1 | 250 mm | 5 µm | Agilent Bio SAX | EK | |
| 5190-2431 | 2.1 | 250 mm | 5 µm | Agilent Bio SCX | EK | |
| 5190-2432 | 2.1 | 50 mm | 5 µm | Agilent Bio SCX | EK | 1/pk |
| 5190-2460 | 2.1 | 50 mm | 10 µm | Agilent Bio WCX | EK | 1/pk |
| 5190-2459 | 2.1 | 250 mm | 10 µm | Agilent Bio WCX | EK | |
| 5190-2452 | 2.1 | 50 mm | 5 µm | Agilent Bio WCX | EK | 1/pk |
| 5190-2451 | 2.1 | 250 mm | 5 µm | Agilent Bio WCX | EK | |
| 5190-2491 | 2.1 | 250 mm | 5 µm | Agilent Bio WAX | EK | |
| 5190-2492 | 2.1 | 50 mm | 5 µm | Agilent Bio WAX | EK | 1/pk |
| 5190-2499 | 2.1 | 250 mm | 10 µm | Agilent Bio WAX | EK | |
| 5190-2440 | 2.1 | 50 mm | 10 µm | Agilent Bio SCX | EK | 1/pk |
| 5190-2480 | 2.1 | 50 mm | 10 µm | Agilent Bio SAX | EK | 1/pk |
| 5190-2500 | 2.1 | 50 mm | 10 µm | Agilent Bio WAX | EK | 1/pk |
| 5190-2439 | 2.1 | 250 mm | 10 µm | Agilent Bio SCX | EK | |
| 5190-2474 | 4 | 10 mm | 10 µm | Agilent Bio SAX | Stainless Steel | 1/pk |
| 5190-2426 | 4 | 10 mm | 5 µm | Agilent Bio SCX | Stainless Steel | 1/pk |
| 5190-2434 | 4 | 10 mm | 10 µm | Agilent Bio SCX | Stainless Steel | 1/pk |
| 5190-2466 | 4 | 10 mm | 5 µm | Agilent Bio SAX | Stainless Steel | 1/pk |
| 5190-2464 | 4 | 10 mm | 3 µm | Agilent Bio SAX | Stainless Steel | 1/pk |
| 5190-2462 | 4 | 10 mm | 1.7 µm | Agilent Bio SAX | Stainless Steel | 1/pk |
| 5190-2422 | 4 | 10 mm | 1.7 µm | Agilent Bio SCX | Stainless Steel | 1/pk |
| 5190-2424 | 4 | 10 mm | 3 µm | Agilent Bio SCX | Stainless Steel | 1/pk |
| 5190-2454 | 4 | 10 mm | 10 µm | Agilent Bio WCX | Stainless Steel | 1/pk |
| 5190-2494 | 4 | 10 mm | 10 µm | Agilent Bio WAX | Stainless Steel | 1/pk |
| 5190-2442 | 4 | 10 mm | 1.7 µm | Agilent Bio WCX | Stainless Steel | 1/pk |
| 5190-2482 | 4 | 10 mm | 1.7 µm | Agilent Bio WAX | Stainless Steel | 1/pk |
| 5190-2446 | 4 | 10 mm | 5 µm | Agilent Bio WCX | Stainless Steel | 1/pk |
| 5190-2444 | 4 | 10 mm | 3 µm | Agilent Bio WCX | Stainless Steel | 1/pk |
| 5190-2486 | 4 | 10 mm | 5 µm | Agilent Bio WAX | Stainless Steel | 1/pk |
| 5190-2484 | 4 | 10 mm | 3 µm | Agilent Bio WAX | Stainless Steel | 1/pk |
| 5190-2428 | 4.6 | 50 mm | 5 µm | Agilent Bio SCX | EK | 1/pk |
| 5190-2473 | 4.6 | 250 mm | 10 µm | Agilent Bio SAX | Stainless Steel | |
| 5190-2427 | 4.6 | 250 mm | 5 µm | Agilent Bio SCX | EK | |
| 5190-2476 | 4.6 | 50 mm | 10 µm | Agilent Bio SAX | EK | |
| 5190-2475 | 4.6 | 250 mm | 10 µm | Agilent Bio SAX | EK | |
| 5190-2435 | 4.6 | 250 mm | 10 µm | Agilent Bio SCX | EK | |
| 5190-2436 | 4.6 | 50 mm | 10 µm | Agilent Bio SCX | EK | 1/pk |
| 5190-2433 | 4.6 | 250 mm | 10 µm | Agilent Bio SCX | Stainless Steel | |
| 5190-2468 | 4.6 | 50 mm | 5 µm | Agilent Bio SAX | EK | |
| 5190-2467 | 4.6 | 250 mm | 5 µm | Agilent Bio SAX | EK | |
| 5190-2465 | 4.6 | 250 mm | 5 µm | Agilent Bio SAX | Stainless Steel | |
| 5190-2463 | 4.6 | 50 mm | 3 µm | Agilent Bio SAX | Stainless Steel | |
| 5190-2461 | 4.6 | 50 mm | 1.7 µm | Agilent Bio SAX | Stainless Steel | |
| 5190-2423 | 4.6 | 50 mm | 3 µm | Agilent Bio SCX | Stainless Steel | |
| 5190-2425 | 4.6 | 250 mm | 5 µm | Agilent Bio SCX | Stainless Steel | |
| 5190-2421 | 4.6 | 50 mm | 1.7 µm | Agilent Bio SCX | Stainless Steel | |
| 5190-2453 | 4.6 | 250 mm | 10 µm | Agilent Bio WCX | Stainless Steel | |
| 5190-2456 | 4.6 | 50 mm | 10 µm | Agilent Bio WCX | EK | 1/pk |
| 5190-2455 | 4.6 | 250 mm | 10 µm | Agilent Bio WCX | EK | |
| 5190-2493 | 4.6 | 250 mm | 10 µm | Agilent Bio WAX | Stainless Steel | |
| 5190-2495 | 4.6 | 250 mm | 10 µm | Agilent Bio WAX | EK | |
| 5190-2496 | 4.6 | 50 mm | 10 µm | Agilent Bio WAX | EK | |
| 5190-6875 | 4.6 | 150 mm | 3 µm | Agilent Bio WAX | Stainless Steel | |
| 5190-2448 | 4.6 | 50 mm | 5 µm | Agilent Bio WCX | EK | 1/pk |
| 5190-2443 | 4.6 | 50 mm | 3 µm | Agilent Bio WCX | Stainless Steel | |
| 5190-2441 | 4.6 | 50 mm | 1.7 µm | Agilent Bio WCX | Stainless Steel | |
| 5190-2447 | 4.6 | 250 mm | 5 µm | Agilent Bio WCX | EK | |
| 5190-2483 | 4.6 | 50 mm | 3 µm | Agilent Bio WAX | Stainless Steel | |
| 5190-2445 | 4.6 | 250 mm | 5 µm | Agilent Bio WCX | Stainless Steel | |
| 5190-2481 | 4.6 | 50 mm | 1.7 µm | Agilent Bio WAX | Stainless Steel | |
| 5190-2487 | 4.6 | 250 mm | 5 µm | Agilent Bio WAX | EK | |
| 5190-2485 | 4.6 | 250 mm | 5 µm | Agilent Bio WAX | Stainless Steel | |
| 5190-2488 | 4.6 | 50 mm | 5 µm | Agilent Bio WAX | EK | |
| 5190-6876 | 10 | 250 mm | 5 µm | Agilent Bio WAX | Stainless Steel | |
| 5190-6878 | 10 | 250 mm | 5 µm | Agilent Bio SCX | Stainless Steel | |
| 5190-6882 | 10 | 250 mm | 5 µm | Agilent Bio SAX | Stainless Steel | |
| 5190-6880 | 10 | 250 mm | 5 µm | Agilent Bio WCX | Stainless Steel | |
| 5190-6879 | 21.2 | 250 mm | 5 µm | Agilent Bio SCX | Stainless Steel | |
| 5190-6877 | 21.2 | 250 mm | 5 µm | Agilent Bio WAX | Stainless Steel | |
| 5190-6883 | 21.2 | 250 mm | 5 µm | Agilent Bio SAX | Stainless Steel | |
| 5190-6881 | 21.2 | 250 mm | 5 µm | Agilent Bio WCX | Stainless Steel |