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清華大學免疫學醫學院轉染BMDMs細胞發表文章到JEM已見刊
閱讀：1496 發布時間：2020-6-15
一、2020一季度，清華大學免疫學與醫學院；清華大學生命科學學院聯合發表標題為：The colonic macrophage transcription factor RBP-J orchestrates intestinal immunity against bacterial pathogens的文章到JEM；
二、用invigentech（英克)INVI DNA RNA Transfection Reagent轉染試劑；
三、成功轉染BMDMs細胞(小鼠骨髓來源的巨噬細胞)發表文章見刊；
四、文章簡介：結腸巨噬細胞轉錄因子RBP-J協調腸道對細菌的免疫病原體研究.
五、文章部分內容見下：
RNA-mediated interference
siRNA specifically targeting mouse Cebpb and nontargeting control siRNA were purchased from GenePharma Company. siRNA was transfected into BMDMs using INVI DNA RNA Transfection Reagent according to the manufacturer’s in structions (Invigentech). Cells were used 48 h after transfection.