細胞名稱

小鼠單核巨噬細胞白血病細胞RAW 264.7

貨物編碼

BFN607200597

產品規格

T25培養瓶x1

1.5ml凍存管x2

細胞數量

1x10^6

1x10^6

保存溫度

27℃

-198℃

運輸方式

常溫保溫運輸

干冰運輸

安全等級

1

用途限制

僅供科研用途               1類

培養體系

DMEM高糖培養基+20%FBS+1%三抗

培養溫度

37℃

二氧化碳濃度

5%

簡介

小鼠單核巨噬細胞白血病細胞RAW 264.7細胞株源自BALB/c小鼠由Abelson鼠科白血病病毒誘導的腫瘤。可產生溶菌酶；sIg-,Ia-，Thy-1.2-。為檢測到病毒顆粒的分泌，XC斑點形成試驗陰性。該細胞可以胞飲中性紅并吞噬乳膠顆粒與酵母聚糖；可以經抗體依賴分裂綿羊紅細胞與腫瘤靶細胞；LPS或PPD處理2天可誘導分裂紅細胞，但對腫瘤靶細胞無作用。小鼠單核巨噬細胞白血病細胞RAW 264.7細胞引種自ATCC(TIB-71)

注釋

Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).

Doubling time: 11 hours (from cell counting), 12 hours (from absorbance) (DOI=10.5897/IJBMBR2013.0154); ~30 hours (CLS).

Transformant: NCBI_TaxID; 11788; Abelson mouse leukemia virus (AbMuLV).

Omics: Metabolome analysis.

Omics: Phagosome quantitative phosphoproteome analysis.

Omics: Phagosome analysis by proteomics.

Omics: SNP array analysis.

Miscellaneous: PubMed=23430347 has a different value for STR 6-4 (17) than that of NIST (18) due to a change in the marker motif (personal communication of Almeida J.L.).

Misspelling: RAW 267.4; Occasionally.

Misspelling: Raw 267.4; Occasionally.

Misspelling: RAW267.4; Occasionally.

Misspelling: RAW 274; Occasionally.

Misspelling: RAW-274; Occasionally.

Misspelling: RAW274; Occasionally.

Derived from sampling site: Ascites.

STR信息

/

參考文獻

PubMed=23430347; DOI=10.1007/s10616-013-9545-7

Almeida J.L., Hill C.R., Cole K.D.

Mouse cell line authentication.

Cytotechnology 66:133-147(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847

Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, de Villena F.P.

SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847-847(2014)

PubMed=25504905; DOI=10.1002/pmic.201400431

Guo M., Hartlova A., Dill B.D., Prescott A.R., Gierlinski M., Trost M.

High-resolution quantitative proteome analysis reveals substantial differences between phagosomes of RAW 264.7 and bone marrow derived macrophages.

Proteomics 15:3169-3174(2015)

PubMed=29889899; DOI=10.1371/journal.pone.0198943

Taciak B., Bialasek M., Braniewska A., Sas Z., Sawicka P., Kiraga L., Rygiel T., Krol M.

Evaluation of phenotypic and functional stability of RAW 264.7 cell line through serial passages.

PLoS ONE 13:E0198943-E0198943(2018)

PubMed=31220119; DOI=10.1371/journal.pone.0218412

Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)