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PureCube Ni-NTA Agarose 蛋白純化瓊脂糖
PureCube His Affinity Agarose (NTA)是針對組氨酸標簽的親和層析產品，組氨酸標簽是應用廣泛的親和層析標簽，這種標簽具有小尺寸、低免疫原性的特點和在多種天然或變性條件下的廣泛適用性，NTA相對于IDA具有更高的洗脫液純度。
產品優勢：
1）德國制造，品質與信譽的堅實保證；
2）結合載量可高達80mg/mL，目前市面上高結合能力；
3）更*的DTT和EDTA穩定性和耐受性；
4）凝膠的多孔性和均勻粒徑，使蛋白與凝膠之間有很好地相互作用；
5）適合低壓環境下的純化過程（大規模純化制備或預裝柱均可使用）；
6）高性價比，為研發、生產降低成本；
7）適用于大腸桿菌和真核細胞的裂解液和上清液；
8）同時可裝載Co, Cu, Zn和其它金屬離子。
優勢詳解：
1. 收率高，純度高
Fig. 1: Over 20% more yield obtained with PureCube Ni-NTA Agarose. SDS-PAGE of GFP expressed in E. coli and purified in gravity columns with PureCube Ni-NTA Agarose and Ni-NTA resin from Competitor Q. 80 mg/mL protein yield was obtained with PureCube Ni-NTA Agarose (E1–E4, Cube) compared to 65 and 48 mg/mL, respectively, with the widely used alternative resins G and Q (E1–E4, Competitor G / Competitor Q).
Cube Biotech | |
蛋白結合能力 | > 80mg/mL |
2. *的DTT和EDTA穩定性
Fig. 2: NTA is robust in the presence of reducing and chelating agents. GFP-His was purified on gravity columns containing PureCube Ni-NTA Agarose after exposing the resin for 1 h to 3 concentrations of DTT or EDTA. NTA exhibits a shallow decay rate in binding capacity.
PureCube Ni-NTA瓊脂糖在DTT和EDTA存在下非常堅實，在穩定性測試中，將PureCube Ni-NTA瓊脂糖置于DTT與EDTA濃度逐漸升高的環境中處理1小時。再用處理過的凝膠在重力層析柱中純化大腸桿菌表達的GFP-His，凝膠的蛋白結合能力因存在DTT與EDTA而下降，但是衰減率平緩，即使在1.5 mM EDTA，凝膠依然具有其結合能力的54%。
Cube Biotech | |
DTT穩定性 | <10mM |
EDTA穩定性 | <1.5mM |
產品特性：
應用 | Specific binding and purification of 6x his-tagged proteins |
特異性 | Affinity to His-tagged proteins |
結合能力 | >80?mg/mL |
Bead Ligand | Ni-NTA |
粒徑大小 | 40 μm |
螯合劑穩定性 | Stable in buffer containing 10 mM DTT and 1 mM EDTA |
保存 | Delivered as a 50?% suspension |
所需試劑耗材 | Lysis Buffer Wash Buffer Elution Buffer Ice bath Refrigerated centrifuge for 50?mL tube (min 10,000 x g) 50?mL centrifuge tube Micropipettor and Micropipetting tips Disposable gravity flow columns with capped bottom outlet, 2 ml ?pH meter End-over-end shaker SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment |
訂貨信息：
31101 | PureCube Ni-NTA Agarose (1 ml) |
31103 | PureCube Ni-NTA Agarose (10 ml) |
31105 | PureCube Ni-NTA Agarose (50 ml) |
31110 | PureCube Ni-NTA Agarose (250 ml) |
31112 | PureCube Ni-NTA Agarose (500 ml) |
31115 | PureCube Ni-NTA Agarose (1000 ml) |
31120 | PureCube Ni-NTA Agarose (5000 ml) |
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目前，成都正民德思生物科技有限公司作為德國蛋白純化產品生產商/服務商Cube Biotech生物科技公司中國總代理、西班牙生物磁性分離設備制造商SEPMAG中國總代理、西班牙免疫診斷試劑研發服務商Pragmatic Diagnostics公司的中國戰略合作方，在成都成立Cube Biotech、SEPMAG和Pragmatic Diagnostics的中國總部辦事處，全權負責在中國地區的營銷事務、銷售及售后服務等所有工作。