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Anti-Rabbit and Mouse HRP-DAB IHC detection kit utilized the newest biotin-free polymerization technology to prepare super sensitive polymeric peroxidase linked conjugates. It utilizes a novel controlled and compact enzyme polymerization technology to achieve higher sensitivity without background. It provides on step detection protocol, superior sensitivity and specificity, short incubation time and faster turnaround.
Prior to staining the formalin-fixed paraffin tissue sections should be deparaffinized and hydrated following heat-induced epitope retrieval or enzyme pretreatment. Endogenous peroxidase should be blocked. Then add primary antibody and incubate at optimal titration and condition. After that add OneStep polymer HRP Goat anti-Rabbit and Mouse IgG （H+L） （specific for IHC） and incubate for?15-30 minutes. Polymer HRP will catalyze DAB to form visible brown deposit at the antigen site. When color development achieved satisfactory level, the slides are washed in water to stop reaction. The stained slides may be mounted with either aqueous mounting or organic mounting medium.