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Recombinant human rhinovirus（HRV）3C protease is widely used in the purification and productions of proteins due to its good stability，high specificity，and enzymatic activity．3C protease is one of the non—structural proteins of HRV，and plays important roles in the life cycle of HRV．This protease specifically recognizes the sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, and cleaves the peptide bond between Gln and Gly.