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Moloney Murine Leukemia Virus Reverse Transcriptase （M-MLV RT） is an RNA-dependent DNA polymerase. The enzyme can use RNA （when synthesizing cDNA） or single-stranded DNA as a template and initiate the synthesis of a complementary DNA strand from a primer. M-MLV reverse transcriptase has no 3′ → 5′ exonuclease activity.