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文獻名： Nanomaterial LaNiTiO3-Fe3O4-based Peroxidase Biomimetic Sensor with High Sensitivity

 

 

作者： Xu, Yanhong; Sun, Ying; Feng, Qiao

Department of Materials Engineering, Xuzhou College of Industrial Technology, Xuzhou, Jiangsu Province,Chinar

 

 

摘要：Background: Hydrogen peroxide (H₂O₂) is widely used in various fields and it plays a quintessential role in a variety of biomolecular processes. H2O2 concentration level is an essential biological parameter in monitoring and maintaining the physiological balance of a living cell, and its variation causes some related diseases. Therefore, it is extremely significant to fabricate biosensor with low cost, which can quickly, accurately and sensitively detect H₂O₂ in a wide range. The aims of this paper are to explore a novel electrochemical sensor with high intrinsic peroxidase-like activity, high sensitivity and stability to effectively detect H₂O₂ concentration in real samples.

Methods: The chemically modified electrode LaNiTiO₃-Fe₃O₄/GCE was fabricated based on the nanomaterial LaNiTiO₃-Fe₃O₄ by a simple process, and its electrochemical properties were investigated in the supporting electrolyte of 0.1 M NaOH by the techniques of cyclic voltammetry and currenttime curves on an electrochemical workstation with a conventional three-electrode system.

Results: LaNiTiO₃-Fe₃O₄ nanoparticles show good peroxidase-like activity for H2O2 at a low applied potential of +0.50 V. Under the optimum conditions, the peroxidase biomimetic sensor LaNiTiO₃-Fe₃O₄/GCE exhibited a wide linear response for H₂O₂ oxidation in the range of 0.05 μM - 3.0 mM (R = 0.9994) with a high sensitivity of 3946.2 μA·mM-1·cm-2 and fast response time of 2 s; besides, the detection limit of H₂O₂ was found to be ca. 5.15 nM (S/N = 3). Moreover, the biosensor exhibited good repeatability, stability and anti-interference. Satisfactory results were obtained when the sensor LaNiTiO₃-Fe₃O₄/GCE was applied to determine H₂O₂ in real samples. All of these results provide support to practical application.

Conclusion: A highly sensitive peroxidase biomimetic sensor based on LaNiTiO₃-Fe₃O₄ with nanoscaled material has been successfully explored, and shows good activity for H₂O₂. The proposed biosensor with simple and low cost exhibited excellent advantages of quick response, wide linear range, low detection limit, high sensitivity, long-term stability and good anti-interference ability, which offer promising applications.

 

關鍵詞：Amperometric biosensor; biomimetic sensor; nanoparticles; perovskite; peroxidase-like; spinel