作者： J.M. Montes de Oca-Ávalosa. R.J. Candalb. M.L.Herreraa.

a

Institute of Polymer Technology and Nanotechnology, University of Buenos Aires-CONICET, Faculty of Engineering, Las Heras 2214, C1127AAQ Ciudad de Buenos Aires, Argentina

b

Instituto de Investigación e Ingeniería Ambiental, Universidad Nacional de San Martín (UNSAM), Campus Miguelete, 25 de Mayo y Francia, 1650 San Martín, Provincia de Buenos Aires, Argentina

摘要：Nanoemulsions stabilized by sodium caseinate (NaCas) were prepared using a combination of a high-energy homogenization and evaporative ripening methods. The effects of protein concentration and sucrose addition on physical properties were analyzed by dynamic light scattering (DLS), Turbiscan analysis, confocal laser scanning microscopy (CLSM) and small angle X-ray scattering (SAXS). Droplets sizes were smaller (~ 100 nm in diameter) than the ones obtained by other methods (200 to 2000 nm in diameter). The stability behavior was also different. These emulsions were not destabilized by creaming. As droplets were so small, gravitational forces were negligible. On the contrary, when they showed destabilization the main mechanism was flocculation. Stability of nanoemulsions increased with increasing protein concentrations. Nanoemulsions with 3 or 4 wt% NaCas were slightly turbid systems that remained stable for at least two months. According to SAXS and Turbiscan results, aggregates remained in the nano range showing small tendency to aggregation. In those systems, interactive forces were weak due to the small diameter of flocs.