文獻名：Synthesis of silver and silver@ zero valent iron nanoparticles using Chromolaena odorata phenolic extract for antibacterial activity and hydrogen peroxide detection

作者： Titilope John Jayeoye^abc，Fredrick Nwude Eze^d，Oladipupo Odunayo Olatunde^e，Soottawat Benjakul^e，Thitima Rujiralai^ab

^a Center of Excellence for Innovation in Chemistry and Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand

^b Analytical Chemistry and Environment Research Unit, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand

^c Department of Chemistry, Biochemistry and Molecular Biology, Alex Ekwueme Federal University, Ndufu Alike-Ikwo, Abakaliki, Ebonyi State, Nigeria

^d Drug Delivery System Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand

^e International Center of Excellence in Seafood Science and Innovation, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand

摘要：A highly efficacious green reductant, Chromolaena odorata phenolic extract (COPE), was used for the biosynthesis of silver nanoparticles (AgNPs) and iron was further in situ deposited to form silver@zero valent iron nanoparticles (Ag@ZVI NPs). Phenolic compounds were extracted from Chromolaena odorata leaves using our carefully formulated method and was further validated by ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. COPE was then used as a green bio-resource for room temperature synthesis of AgNPs. After successful synthesis of AgNPs, ferric (III) chloride was then added to realize Ag@ZVI NPs. X-ray diffraction validated the growth of Fe⁰ on the (111) crystalline facet of AgNPs during the formation of Ag@ZVI NPs. The synthesized AgNPs and Ag@ZVI NPs are highly stable, spherical and dispersed, with demonstrable antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Staphylococcus aureus and Listeria monocytogenes. The minimum inhibitory concentration and minimum bactericidal concentration ranged from 1 to 4 μg/mL and 2–8 μg/mL, respectively. Additionally, the antioxidant studies were probed by the DPPH-RSA, ABTS-RSA and MCA, which all confirmed the ability of the biosynthesized nanomaterials to scavenge radicals. The colorimetric sensing ability of COPE AgNPs was tested against hydrogen peroxide (H₂O₂) by visual observation and UV–vis spectrophotometry. A wide linear range from 5 to 200 µM as well as a low detection limit of 1.46 µM H₂O₂ was achieved. The practicality of the AgNPs sensor towards H₂O₂ was successfully performed on water sample with reliable accuracy.

關鍵詞：Silver nanoparticles；Zero valent iron；Chromolaena odorata；Phenolic extract；Nanotechnology；Sensor