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測量應用案例-20211203

來源:美國布魯克海文儀器公司   2021年12月08日 10:59  
 

文獻名: Visual and colorimetric detection of uric acid in human serum and urine using chitosan stabilized gold nanoparticles

 

 

作者 Fan Lia, Tianshuang Hea, Sihao Wua, Zoujun, Penga Ping Qiuab, Xiaomin Tangc

aDepartment of Chemistry, Nanchang University, Nanchang 330031, China

bJiangxi Province Key Laboratory of Modern Analytical Science, Nanchang University, Nanchang 330031, China

cThe Fourth Affiliated Hospital, Nanchang University, Nanchang 330003, Jiangxi, China

 

 

摘要:In this paper, the chitosan stabilized gold nanoparticles was developed as a catalyzed 3,3′,5,5′-tetramethylbenzidine - H2O2 system for visual and colorimetric detection of uric acid. In theory, uric acid can be catalyzed by uricase to produce H2O2, and the chitosan stabilized gold nanoparticles were successfully synthesized by chemical reduction and showed an intrinsic peroxidase-like activity. The chitosan stabilized gold nanoparticles could effectively catalyze decomposition of H2O2 to generate OH radicals. The peroxidase substrate 3,3′,5,5′-tetramethylbenzidine is oxidized in the presence of OH radicals, resulting in a visible color change (from colorless to blue). This convenient, novel and sensitive method could be used for detection of uric acid based on the chitosan stabilized gold nanoparticles, with a linear range from 0.1 to 30 μM, and a limit of detection (S/N ratio of 3) as low as 0.04 μM. Furthermore, the detection method has been successfully applied to human serum and urine samples by visual observation. The recoveries of uric acid in human serum and urine were in the range of 96.1–103.1% and 95.2–97.7%, respectively. These results revealed that the visual colorimetric method may provide an alternative to clinical medicine and daily monitoring.

 

關鍵詞:Uric acid; Chitosan stabilized gold nanoparticles; 3,3′,5, 5′-Tetramethylbenzidine; Uricase; Serum and urine samples

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