Absin ECL化學發光檢測試劑盒產品詳情

1. 基本屬性

中文名稱	ECL化學發光檢測試劑盒
英文名字	ECL luminescence reagent
別名	ECL發光液；HRP化學發光底物
產品貨號	abs920
產品規格	2*25ml，2*50ml，2*250ml
產品組份	A液；B液
保存建議	2-8℃避光保存6個月。
產品說明書

2. 產品描述

Western blot底物發光檢測試劑可由標記于二抗上的辣根過氧化酶催化，產生化學發光反應，可以靈敏地檢測出目的蛋白的存在。ECL底物化學發光檢測試劑盒基于新一代增強型化學發光底物研制而成，并對成份做了優化。產品背景低，穩定性好。它由辣根過氧化物酶(HRP)催化發生化學反應，發出熒光，可對X光膠片曝光，也可直接進行luminometer檢測或者熒光CCD掃描。

3. 操作步驟

1. 按常規Western blot操作，二抗孵育后，進行最后一次洗滌時，根據膜的大小，按每10 cm2 膜混合0.5ml溶液A和0.5ml溶液B混勻，配制成發光檢測工作液。 
2. 用平頭鑷子將膜取出，膜的下緣輕輕接觸吸水紙，以去除膜上多余的液體。膜的蛋白面朝上，置于潔凈保鮮膜（某些市售保鮮膜包裹印跡膜時可能會淬滅熒光，應選擇高質量保鮮膜）上。用吸管將配制的發光檢測液轉移到蛋白膜上，使其均勻覆蓋，室溫孵育2分鐘。 
3. 用平頭鑷夾持蛋白膜，膜的下緣輕輕接觸吸水紙，以去除膜上多余的液體。膜的蛋白面朝上，包裹于潔凈保鮮膜內。輕輕趕出其間的氣泡，固定在X片暗盒內。
4. 在暗室中取一張X片置于包裹的膜上，合上暗盒，曝光30秒至1分鐘。立即顯影定影，根據其曝光強度，縮短或延長下一張X片的曝光時間（對微弱信號，曝光時間可延長至數小時）。

4. 用法說明

1.ECL A液和B液在吸取過程中必須要更換槍頭，A液和B液相互污染后會導致 A液或B液逐漸失效，影響后續的使用效果。
2.各溶液使用后，請蓋緊瓶蓋，以防失效。特別是B液，含有氧化劑，比較容易 被還原而失效。
3.ECL的熒光持續時間很長，但開始反應后的30分鐘內熒光更強一些，隨后熒光會逐漸減弱，因此請注意充分利用這熒光較強的30分鐘進行壓片。 
4.ECL A液和B液均對人體有害，操作時請注意適當防護。 為了您的安全和健康，請穿實驗服并戴一次性手套操作。

ECL化學發光檢測試劑盒部分文獻展示

• Silencing Long Non-coding RNA Kcnq1ot1 Limits Acute Kidney Injury by Promoting miR-204-5p and Blocking the Activation of NLRP3 Inflammasome

  JunTao Wang, Peng Jiao, XiaoYing Wei, Yun Zhou

  Frontiers in Physiology. 2021 Nov 11 .

  影響因子： 4.134

• Capsaicin restores sodium iodine symporter-mediated radioiodine uptake through bypassing canonical TSH?TSHR pathway in anaplastic thyroid carcinoma cells

  Shichen Xu, Xian Cheng, Jing Wu, Yunping Wang, Xiaowen Wang, Liying Wu, Huixin Yu, Jiandong Bao, Li Zhang

  Journal of Molecular Cell Biology. 2021 Nov 09 .

  影響因子： 6.216

• Knockdown of long non-coding RNA AGAP2-AS1 suppresses the proliferation and metastasis of glioma by targeting microRNA-497-5p

  Yi Sun,Yulong Shen,Xing LiORCID Icon

  Bioengineered. 2021 Oct 28 .

  影響因子： 3.269

• Potentilla discolor ameliorates LPS-induced inflammatory responses through suppressing NF-κB and AP-1 pathways

  Xiaoyu Zhang,Yuan Kang,Ximeng Li,Yunfeng Huang,Ruijuan Qi,Yixin Han,Runlan Cai,Yuan Gao,YunQi

  Biomedicine & Pharmacotherapy. 2021 Oct 19 .

  影響因子： 6.529

• Caffeine‐induced neurotoxicity mediated by Nrf2 pathway in PC12 cells and zebrafish larvae

  S Chian, Z Jiang, L Jiang, K Wang

  JOURNAL OF APPLIED TOXICOLOGY. 2021 Sep 29 .

  影響因子： 3.446

• Cancer-Derived Exosomal miR-651 as a Diagnostic Marker Restrains Cisplatin Resistance and Directly Targets ATG3 for Cervical Cancer

  Xiaofan Zhu, Ling Long, He Xiao,Xuan He

  diseases markers. 2021 Sep 19 .

  影響因子： 3.434

• Sophoraflavanone M, a prenylated flavonoid from Sophora flavescens Ait., suppresses pro-inflammatory mediators through both NF-κB and JNK/AP-1 signaling pathways in LPS-primed macrophages

  Yixin Han, Xiaoyu Zhang, Yuan Kang, Yuan Gao, Ximeng Li, Ruijuan Qi, Runlan Cai, Yun Qi

  European Journal of Pharmacology. 2021 Sep 15 .

  影響因子： 3.100

• Sphingosine-1-phosphate induces myocyte autophagy after myocardial infarction through mTOR inhibition

  Li-Guo Yang, Ai-Ling Wang, Lu Li, Hong Yang, Xi Jie, Zong-Feng Zhu, Xiao-Juan Zhang, Hui-Ping Zhao, Rui-Fang Chi, Bao Li, Fu-Zhong Qin, Jia-Pu Wang, Ke Wang

  European Journal of Pharmacology. 2021 Sep 15 .

  影響因子： 4.432

• Role of caveolin?1 in chronic postsurgical pain in rats

  Sai-Sai, Huang Su, Cao Yi-Bin, Qin Cui, E. Lu, Shi-Ren Shen

  Experimental and Therapeutic Medicine. 2021 Sep 13 ; 5

  影響因子： 2.447

• The Disruption of the Endothelial Barrier Contributes to Acute Lung Injury Induced by Coxsackievirus A2 Infection in Mice

  Wangquan Ji,Qiang Hu,Mengdi Zhang,Chuwen Zhang ,Chen Chen,Yujie Yan,Xue Zhang,Shuaiyin Chen,ORCID,Ling Tao,Weiguo Zhang,Yuefei Jin,ORCID andGuangcai Duan

  INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2021 Sep 13 .

  影響因子： 5.923

• Overexpression of RAB34 Associates With Tumor Aggressiveness and Immune Infiltration in Glioma

  Peng Hou, Quan Wan, Qing Wang, Xuechao Wu, Xiaojie Lu

  Research Square. 2021 Aug 17 .

• Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model

  Wangquan Ji, Peiyu Zhu, Ruonan Liang, Liang Zhang, Yu Zhang, Yuexia Wang, Weiguo Zhang, Ling Tao, Shuaiyin Chen, Haiyan Yang, Yuefei Jin, Guangcai Duan

  Viruses. 2021 Aug 11 ; 8

  影響因子： 5.048

• Long non-coding RNA Gm11974 aggravates oxygen–glucose deprivation-induced injury via miR-122-5p/SEMA3A axis in ischaemic stroke

  Ling Yang, Lijuan Wang, Juan Wang, Ping Liu

  Metabolic Brain Disease . 2021 Aug 02 .

  影響因子： 2.638

• HMGB1介導中性粒細胞自噬調控1,3-β-葡聚糖所致肺部炎癥的作用機制

  劉凱悅

  . 2021 Jun 30 .

• PAQR3逆轉非小細胞肺癌A549/DDP細胞順鉑耐藥機制研究

  方世旭

  遵義醫科大學. 2021 Jun 30 .

• Hepatotoxicity of copper sulfide nanoparticles towards hepatocyte spheroids using a novel multi-concave agarose chip method

  Tianyan Jiang, Haoxiang Guo, Ya-Nan Xia, Yun Liu, Dandan Chen, Guibin Pang, Yahui Feng, Huan Yu, Yanxian Wu, Shaodian Zhang, Yangyun Wang, Yong Wang, Hairuo Wen, Leshuai W Zhang

  Future Medicine. 2021 Jun 29 .

  影響因子： 4.018

• LncRNA CASC2 Alleviates Sepsis-induced Acute Lung Injury by Regulating the miR-152-3p/PDK4 Axis

  Lili Zhu,Dongwu Shi,Jianghong Cao,Lu Song

  Immunological Investigations. 2021 Jun 24 .

  影響因子： 2.385

更多ECL化學發光液相關文獻>>>