PureCube His Affinity Agarose (NTA)是針對組氨酸標簽的親和層析產品，組氨酸標簽是應用廣泛的親和層析標簽，這種標簽具有小尺寸、低免疫原性的特點和在多種天然或變性條件下的廣泛適用性，NTA相對于IDA具有更高的洗脫液純度。

產品優勢：

1）德國制造，品質與信譽的堅實保證；

2）結合載量可高達80mg/mL，目前市面上高結合能力；

3）更*的DTT和EDTA穩定性和耐受性；

4）凝膠的多孔性和均勻粒徑，使蛋白與凝膠之間有很好地相互作用；

5）適合低壓環境下的純化過程（大規模純化制備或預裝柱均可使用）；

6）高性價比，為研發、生產降低成本；

7）適用于大腸桿菌和真核細胞的裂解液和上清液；

8）同時可裝載Co, Cu, Zn和其它金屬離子。

優勢詳解：

1. 收率高，純度高

Fig. 1: Over 20% more yield obtained with PureCube Ni-NTA Agarose. SDS-PAGE of GFP expressed in E. coli and purified in gravity columns with PureCube Ni-NTA Agarose and Ni-NTA resin from Competitor Q. 80 mg/mL protein yield was obtained with PureCube Ni-NTA Agarose (E1–E4, Cube) compared to 65 and 48 mg/mL, respectively, with the widely used alternative resins G and Q (E1–E4, Competitor G / Competitor Q).

2. *的DTT和EDTA穩定性

Fig. 2: NTA is robust in the presence of reducing and chelating agents. GFP-His was purified on gravity columns containing PureCube Ni-NTA Agarose after exposing the resin for 1 h to 3 concentrations of DTT or EDTA. NTA exhibits a shallow decay rate in binding capacity.

PureCube Ni-NTA瓊脂糖在DTT和EDTA存在下非常堅實，在穩定性測試中，將PureCube Ni-NTA瓊脂糖置于DTT與EDTA濃度逐漸升高的環境中處理1小時。再用處理過的凝膠在重力層析柱中純化大腸桿菌表達的GFP-His，凝膠的蛋白結合能力因存在DTT與EDTA而下降，但是衰減率平緩，即使在1.5 mM EDTA，凝膠依然具有其結合能力的54%。

產品特性：

訂貨信息：

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