caspase是一類蛋白酶家族，成員較多，在人類，已經(jīng)鑒定了11種不同的caspase，Caspase-1在介導(dǎo)炎癥反應(yīng)和細(xì)胞焦亡(pyroptosis)中發(fā)揮重要作用。市面上常見的是檢測細(xì)胞裂解液中的Caspase-1活性，更多研究者希望動(dòng)態(tài)檢測活細(xì)胞 內(nèi)的Caspase-1的活性，Immunochemistry ICT FAM-FLICA Caspase-1 活性分析試劑盒能針對(duì)性地活性進(jìn)行定量和監(jiān)測。

艾美捷Immunochemistry 天冬氨酸蛋白酶Caspase-1活性檢測試劑盒原理：

FLICA試劑FAM-YVAD-FMK（Ex/Em = 488nm/530nm），是無細(xì)胞毒性的，細(xì)胞膜滲透抑制劑。進(jìn)入每個(gè)細(xì)胞，并不可逆地與活化的caspase-1結(jié)合。任何未結(jié)合的FAM-YVAD-FMK-FLICA試劑擴(kuò)散出細(xì)胞并被沖走。因此留在細(xì)胞內(nèi)的綠色熒光直接體現(xiàn)了活性caspase-1酶活性的。熒光結(jié)果可通過熒光酶標(biāo)儀、熒光顯微鏡、流式細(xì)胞儀進(jìn)行分析。

艾美捷Immunochemistry 天冬氨酸蛋白酶Caspase-1活性檢測試劑盒組分：

25T：

l FLICA Caspase-1 Reagent (FAM-YVAD-FMK), 1 vial, #653 l 10X Apoptosis Wash Buffer, 15 mL, #635 l Fixative, 6 mL, #636 l Propidium Iodide, 1 mL, #638 l Hoechst 33342, 1 mL, #639 l Kit Manual

100T：

l FLICA Caspase-1 Reagent (FAM-YVAD-FMK), 4 vials, #653 l 10X Apoptosis Wash Buffer, 60 mL, #634 l Fixative, 6 mL, #636 l Propidium Iodide, 1 mL, #638 l Hoechst 33342, 1 mL, #639 l Kit Manual

保存條件：2-8°C 避光保存。

FAM-FLICA Caspase-1 活性分析試劑盒Nature文章鑒賞：

IF=30.9, 《Escherichia coli Rho GTPase-activating toxin CNF1 mediates NLRP3 inflammasome activation via p21-activated kinases-1/2 during bacteraemia in mice》,Nature Microbiology

更多發(fā)表文章，部分：

1、Liang, C;Peng, Y;Sun, H;Wang, L;Jiang, L;Zou, S. Silencing lncRNA KCNQ1OT1 reduced hepatic ischemia reperfusion injury-induced pyroptosis by regulating miR-142a-3p/HMGB1 axis. Molecular and Cellular Biochemistry. 2022 October 29; doi: 10.1007/s11010-022-04586-y. Full Article

2、Jiang, Y;Yang, Y;Hu, Y;Yang, R;Huang, J;Liu, Y;Wu, Y;Li, S;Ma, C;Humphries, F;Wang, B;Wang, X;Hu, Z;Yang, S. Gasdermin D restricts anti-tumor immunity during PD-L1 checkpoint blockade. Cell reports. 2022 October 25; doi: 10.1016/j.celrep.2022.111553. Text

3、Moraes, C;Hottz, E;Dos Santos Ornellas, D;Adesse, D;de Azevedo, C;d’Avila, J;Zaverucha-do-Valle, C;Maron-Gutierrez, T;Barbosa, H;Bozza, P;Bozza, F. Microglial NLRP3 Inflammasome Induces Excitatory Synaptic Loss Through IL-1β-Enriched Microvesicle Release: Implications for Sepsis-Associated Encephalopathy. Molecular Neurobiology. 2022 October 25; doi: 10.1007/s12035-022-03067-z.