CBP73208 | |
| I. Introduction | |
Cell Line Name： | c-Kit Y823D/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
KIT Y823D lies within the protein kinase domain of the Kit protein (UniProt.org). Y823D confers a gain of function on Kit, as indicated by constitutive phosphorylation of Kit in cultured cells (PMID: 14695343, PMID: 20633291). | |
| III. Representative Data | |
1.WB of c-Kit Y823D/BaF3
| |
2.Sanger of c-Kit Y823D/BaF3
| |
3. Anti-proliferation assay
Figure 3. CTG Proliferation Assay of BaF3 c-KIT Y823D (C1). | |
| |
化工儀器網