CBP73294 | |
| I. Introduction | |
Cell Line Name： | c-Kit K642E-D816A/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
KIT K642E lies within the tyrosine kinase domain 1 (exon 13) of the Kit protein (PMID: 15990278). K642E results in constitutive phosphorylation of Kit and activation of downstream signaling (PMID: 15990278, PMID: 19802003), as well as transformation of cultured cells (PMID: 11073817). | |
| III. Representative Data | |
1.Sanger of c-Kit K642E-D816A/BaF3
Figure 1. c-Kit_K642E-D816A/BaF3 K642E
Figure 2. c-Kit K642E-D816A/BaF3 D816A | |
2. Anti-proliferation assay
Figure 3. CTG Proliferation Assay of BaF3 c-KIT K642E/D816A (C5). | |
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