Mouse GCGR CRE-Luc HEK293
| I. Background | |
胰高血糖素是一種胰腺肽激素，作為胰島素的反調(diào)節(jié)激素，刺激葡萄糖通過肝臟釋放， 維持葡萄糖穩(wěn)態(tài)。GCGR 是一種 B 類 GPCR，最初被描述為與腺苷酸環(huán)化酶功能相連的胰 高血糖素結(jié)合體。它在維持葡萄糖穩(wěn)態(tài)中起關(guān)鍵作用，因此被認(rèn)為是治療糖尿病的一個有 價值的靶點。 | |
| II. Description | |
Mouse GCGR CRE-Luc HEK293 報告基因藥靶模型很好的模擬了體內(nèi) GCGR 的信號轉(zhuǎn)導(dǎo)過程，原理見下圖所示。
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| III. Introduction | |
| Expressed gene: | Mouse GCGR |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+200 μg/ml Hygromycin B+2 μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional assay for Mouse GCGR |
| Transducer: | Gs |
IV. Representative Data
Figure 2. Dose Response of Glucagon(1-29) in Mouse GCGR CRE-Luc HEK293(C17). | |
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